Human Genetics

, Volume 92, Issue 6, pp 583–587 | Cite as

Chromosomal localization of 9 KOX zinc finger genes: physical linkages suggest clustering of KOX genes on chromosomes 12, 16, and 19

  • Marie-Françoise Rousseau-Merck
  • Josette Hillion
  • Philippe Jonveaux
  • Philippe Couillin
  • Paule Seité
  • Hans-Jürgen Thiesen
  • Roland Berger
Original Investigations

Abstract

Nine KOX zinc finger genes were localized on four human chromosomes by in situ hybridization of cDNA probes to metaphase chromosomes. KOX1 (ZNF10), KOX11 (ZNF18), and KOX12 (ZNF19) were mapped to chromosome bands 12q24.33, 17p13-p12, and 16q22-q23, respectively. Six other KOX genes were localized on chromosome 19: KOX6 (ZNF14) and KOX13 (ZNF20) to 19p13.3-p13.2, KOX5 (ZNF13) and KOX22 (ZNF27) to 19q13.2-qter, and KOX24 (ZNF28) and KOX28 (ZNF30) to 19q13.4. Pulsed field gel electrophoresis experiments showed that the pairs of KOX genes found on the chromosome bands 12q24.33, 16q22-q23, 19p13.3-p13.2, or 19q13.3-qter lie within 200–300 kb DNA fragments. This suggests the existence of KOX gene clusters on these chromosomal bands.

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References

  1. Aubry M, Marineau C, Zhang FR, Zahed L, Figlewicz D, Delattre O, Thomas G, Jong P de, Julien JP, Rouleau GA (1992) Cloning of six new genes with zinc fingers motifs mapping to short and long arms of human acrocentric chromosomes 22 (p and q11.2). Genomics 13:641–648Google Scholar
  2. Bellefroid EJ, Lecocq PJ, Benhida Q, Poncelet DA, Belayew A, Martial JA (1989) The human genome contains hundreds of genes encoding zinc finger protein of the Krüppel type. DNA 8:377–387Google Scholar
  3. Bellefroid EJ, Marine J-C, Ried T, Lecocq PJ, Riviere M, Amemiya C, Poncelet DA, Colie PG, Jong P de, Szpirer C, Ward DC, Martial JA (1993) Clustered organization of homologous KRAB zinc-finger genes with enhanced expression in human T lymphoid cells. EMBO J 12:1363–1374Google Scholar
  4. Bray P, Thiesen HJ (1990) Putting the finger on the DNA. New Biol 2:325–327Google Scholar
  5. Bray P, Lichter P, Thiesen HJ, Ward D, Dawid IB (1991) Characterization and mapping of human genes encoding zinc finger proteins. Proc Natl Acad Sci USA 88:9563–9567Google Scholar
  6. Cannizzaro LA, Aronson MM, Thiesen HJ (1993) Human zinc finger gene ZNF 23 (KOX 16) maps to a zinc finger gene cluster on chromosome 16q22 and ZNF32 (KOX 30) to chromosome region 10q23-q24. Hum Genet 91:383–385Google Scholar
  7. Chowdhury K, Deutsch U, Gruss P (1987) A multiple family encoding several “finger” structures is present and differentially active in mammalian genomes. Cell 48:771–778Google Scholar
  8. Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13Google Scholar
  9. Ginsberg A, King BO, Roeder RG (1984) Xenopus 5 S gene transcription factor, TF III A: characterization of a cDNA clone and measurement of RNA levels throughout development. Cell 39: 479–489Google Scholar
  10. Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-based human chromosomes by in situ hybridization. Chromosoma 83:431–439Google Scholar
  11. Hoovers JMN, Mannens M, John R, Bliek J, Heyningen V van, Porteous DJ, Leschot NJ, Westerveld A, Little PFR (1992) High-resolution localization of 69 potential human zinc finger protein genes: a number are clustered. Genomics 12:254–263Google Scholar
  12. Huebner K, Druck T, Croce CM, Thiesen HJ (1991) Twenty seven non overlapping zinc finger cDNAs from human T cells map to nine different chromosomes with apparent clustering. Am J Hum Genet 48:726–740Google Scholar
  13. Lichter P, Bray P, Ried T, Dawid I, Ward D (1992) Clustering of C2-H2. Zinc finger motif sequences within telomeric and fragile site regions of human chromosomes. Genomics 13:999–1007Google Scholar
  14. Lovering R, Trowsdale J (1991) A gene encoding 22 highly related zinc fingers is expressed in lymphoid cell lines. Nucleic Acids Res 19:2921–2928Google Scholar
  15. McGinnis W, Krumlauf R (1992) Homeobox genes and axial patterning. Cell 68:283–302Google Scholar
  16. Miller J, McLachlan AD, Klug A (1985) Repetitive zinc binding domains in the protein transcription factor III A from Xenopus oocytes. EMBO J 4:1604–1614Google Scholar
  17. Panutti A, Lanfrancone L, Pascussi A, La Mantia G, Pelicci PG, Nania L (1988) Isolation of cDNA encoding finger proteins and measurements of the corresponding mRNA levels during myeloid terminal differentiation. Nucleic Acids Res 16:4227–4237Google Scholar
  18. Pellegrino GR, Berg JM (1991) Identification and characterization of “zinc finger” domains by the polymerase chain reaction. Proc Natl Acad Sci USA 88:671–675Google Scholar
  19. Rosenberg UB, Schroeder C, Priess A, Kielin A, Cote S, Riede J, Jackle H (1986) Structural homology of the product of the Drosophila Krüppel gene with Xenopus transcription factor III A. Nature 319:336–339Google Scholar
  20. Rousseau-Merck MF, Zahraoui A, Touchot N, Tavitian A, Berger R (1991a) Chromosome assignment of four RAS-related RAB genes. Hum Genet 86:350–354Google Scholar
  21. Rousseau-Merck MF, Huebner K, Berger R, Thiesen HJ (1991b) Chromosomal localization of two human zinc finger protein genes, ZNF24 (KOX 17) and ZNF29 (KOX26), to 18q12 and 17p13-12, respectively. Genomics 9:154–161Google Scholar
  22. Rousseau-Merck MF, Tunnacliffe A, Berger R, Ponder BA, Thiesen HJ (1992) A cluster of expressed zinc finger protein genes in the peri centromeric region of human chromosome 10. Genomics 13:845–848Google Scholar
  23. Saleh M, Selleri L, Little PFR, Evans GA (1992) Isolation and expression of linked zinc finger gene clusters on human chromosome 11q. Genomics 14:970–978Google Scholar
  24. Seite P, Huebner K, Rousseau-Merck MF, Berger R, Thiesen HJ (1991) Two human genes encoding zinc finger proteins genes, ZNF12 (KOX3) and ZNF26 (KOX20), map to chromosomes 7p22-p21 and 12q24.33, respectively. Hum Genet 86:585–590Google Scholar
  25. Thiessen HJ (1990) Multiple genes encoding zinc finger domains re expressed in human T cells. New Biol 2:363–374Google Scholar
  26. Tunnacliffe A, Liu L, Moore JK, Leversha MA, Jackson MS, Papi L, Ferguson-Smith MA, Thiesen HJ, Ponder BAJ (1993) Duplicated KOX zinc finger gene clusters flank the centromere of human chromosome 10: evidence for a pericentric inversion during primate evolution. Nucleic Acids Res 21:1409–1417Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Marie-Françoise Rousseau-Merck
    • 1
  • Josette Hillion
    • 1
  • Philippe Jonveaux
    • 1
  • Philippe Couillin
    • 3
  • Paule Seité
    • 1
  • Hans-Jürgen Thiesen
    • 2
  • Roland Berger
    • 1
  1. 1.INSERM U.301, Institut de Génétique MoléculaireParisFrance
  2. 2.Basel Institute for ImmunologyBaselSwitzerland
  3. 3.INSERM U.178Villejuif CedexFrance

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