Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Liver and intestinal fatty acid binding proteins in control and TGFβ1 gene targeted deficient mice

Abstract

The effect of transforming growth factor beta-1 (TGFβ1) expression on fatty acid binding proteins was examined in control and two strains of gene targeted TGFβ1-deficient mice. Homozygous TGFβ1-deficient 129 × CF-1, expressing multifocal inflammatory syndrome, had 25% less liver fatty acid binding protein (L-FABP) when compared to control mice. The decrease in L-FABP expression was not due to multifocal inflammatory syndrome since homozygous TGFβ1-deficient/immunodeficient C3H mice on a SLID background had 36% lower liver L-FABP than controls. This effect was developmentally related and specific to liver, but not the proximal intestine, where L-FABP is also expressed. Finally, the proximal intestine also expresses intestinal-FABP (1-FABP) which decreased 3-fold in the TGFβ1-deficient/immunodeficient C3H mice only. Thus, TGFβ1 appears to regulate the expression of L-FABP and I-FABP in the liver and the proximal intestine, respectively.

This is a preview of subscription content, log in to check access.

Abbreviations

L-FABP:

liver fatty acid binding protein

I-FABP:

intestinal fatty acid binding protein

TGFβ1:

transforming growth factor beta-1

TNF-α:

tumor necrosis factor-α

MIP-α:

macrophage inflammatory protein-α

PMSF:

phenylmethyl sulfonyl fluoride

PBS:

phosphate buffered saline

References

  1. 1.

    Millan FA, Denkez F, Kondaiah P, Akhurst RJ: Embryonic gene expression patterns ofTGFβ1, β2 and β3 suggest different development functions in vivo. Development 111: 131–144, 1991

  2. 2.

    Roberts AB, Sporn MB: The transforming growth factor βs. In: M.B. Sporn and A.B. Roberts (eds). Peptide Growth Factors and their Receptors. Heidelberg: Springer-Verlag, 1990, pp 419–472

  3. 3.

    Shull MM, Ormsby I, Kier AB, Pawlowski S, Diebold RJ, Yin M, Allen R, Sidman C, Proetzel G, Calvin Dawn, Annunziata N, Doetschman T: Targeted disruption of the murine transforming growth factor β1 gene results in multifocal inflammatory disease. Nature 359: 693–699, 1992

  4. 4.

    Spiegelman BM, Hotamisligil GS: Through thick and thin: Wasting, obesity, and TNFα. Cell 73: 625–627, 1993

  5. 5.

    Stephens JM, Butts M Stone, R Pekala, PH, Bernlohr DA: Regulation of transcriptional factor mRNA accumulation during 3T3-L1 preadipocyte differentiation by antagonists of adipogenesis. Mol Cell Biochem 123: 63–71, 1993

  6. 6.

    Sparks RL, Allen BJ, Straus EE: TGFβ blocks early but not late differentiation specific gene expression and morphologic differentiation of 3T3 T adipocytes. J Cell Physiol 150: 568–577, 1992

  7. 7.

    Richardson, R.L., Campion, D.R., Hausman, G.J., and Wright, J.T.: Transforming growth factor β (TGF β) and adipogenesis in pigs. J Anim Sci 67: 2171–2180, 1989

  8. 8.

    Paulussen RJA, Veerkamp JH: Intracellular fatty acid binding proteins: characteristics and functions. In: H.J. Hilderson (ed.) Subcellular Chemistry (ed.) Vol. 16, Plenum Press, NY, 1990, pp 175–226

  9. 9.

    Prows DR, Murphy EJ, Schroeder F: Intestinal and liver fatty acid binding proteins differentially affect fatty acid uptake and esterification in L-cells. Lipids 30: 907–910, 1995

  10. 10.

    Murphy EJ, Prows DR, Jefferson JR, Schroeder F: Liver fatty acid binding protein expression in transfected fibroblasts stimulates fatty acid uptake. Biochim Biophys Acta, 1996, in press

  11. 11.

    Schroeder F, Jefferson JR, Powell D, Incerpi S, Woodford JK, Colles SM, Myers-Payne S, Emge T, Hubbell T, Moncecchi D, Prows DR, Heyliger CE: Expression of rat L-FABP in mouse fibroblasts: role in fat absorption. Mol Cell Biochem 123: 73–83, 1993

  12. 12.

    Jefferson JR, Powell DM, Rymaszewski Z, Kukowska-Latallo J, Lowe JB, Schroeder F: Altered membrane structure in transfected mouse L-cell fibroblasts expressing rat liver fatty acid binding protein. J Biol Chem 265: 11062–11068, 1990

  13. 13.

    Jefferson JR, Nemecz G, Pastuszyn A, Scallen TJ, Schroeder F: Intracellular sterol distribution in transfected mouse L-cell fibroblasts expressing rat liver fatty acid binding protein. J Biol Chem 266: 5486–5496, 1991

  14. 14.

    Baier LJ, Sachettini JC, Knowler WC, Eads J, Paolisso G, Tataranni PA, Mochizuki H, Bennett PH, Bogardus C, Prochazka M: An amino substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increased fat oxidation, and insulin resistance. J Clin Invest 95: 1281–1287

  15. 15.

    Nemecz G, Schroeder F: Selective binding of cholesterol by recombinant fatty acid binding proteins. J Biol Chem 266: 17180–17186, 1991

  16. 16.

    Nemecz G, Hubbell T, Jefferson JR, Lowe JB, Schroeder F: Interaction of fatty acids with recombinant rat intestinal and liver fatty acid binding proteins. Arch Biochem Biophys 286: 300–309, 1991

  17. 17.

    Boivin GP, O'Toole BA, Ormsby IE, Doetschman T, Kier AB: Onset and progression of pathologic lesions in TGF β1 deficient mice. Am J Path 146: 276–288, 1995

  18. 18.

    Diebold RJ, Eis MJ, Yin M. Ormsby I, Boivin GP, Darrow BJ, Safitz JE, Doetschman T: Early-onset multifocal inflammation in the transforming growth factor β1-null mouse is lymphocyte mediated. Proc Natl Acad Sci USA 92: 12215–12219, 1995

  19. 19.

    Harlow ED, Lane D: Antibodies, A Laboratory Manual. E.D. Harlow and D. Lane (eds). Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1988

  20. 20.

    Schagger H, von Jagow G: Tricine sodium dodecyl sulfate poly acrylamide gel electrophoresis for separation of proteins in the range from 1-100 kDa. Anal Biochem 166: 368–379, 1987

  21. 21.

    Towbin HT, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci 76: 4350–4354, 1979

  22. 22.

    Frolik CA, Dart LL, Meyers DA, Smith DM, Sporn MB: Purification and initial characterization of a type β transforming growth factor from human placenta. Proc Natl Acad Sci USA 80: 3676–3680, 1983

  23. 23.

    Cox, D.A. and Burk, R.R.: Isolation and characterization of milk growt a transforming growth factor β-2 related peptide, from bovine milk. Eur J Biochem 197: 353–358, 1991

  24. 24.

    Bass NM, Manning JA, Ockner RK: Turnover and short term regulation of fatty acid binding protein in liver. J Biol Chem 260: 9603–9607, 1985

Download references

Author information

Correspondence to Ann B. Kier.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Fontaine, R.N., Gossett, R.E., Schroeder, F. et al. Liver and intestinal fatty acid binding proteins in control and TGFβ1 gene targeted deficient mice. Molecular and Cellular Biochemistry 159, 149–153 (1996). https://doi.org/10.1007/BF00420917

Download citation

Key words

  • fatty acid binding protein
  • liver
  • intestine
  • growth factor
  • TGFβ1