UV-induced curing of the double-stranded RNA virus of the yeast Yarrowia lipolytica
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Summary
Curing of the viruslike particles harbored by a strain of the yeast Yarrowia lipolytica was achieved by UV irradiation. The cured strain was found to be able to maintain the viruslike particles after their re-introduction by crossing or by cytoplasmic fusion. The involvement of a UV-induced mutation of a yeast maintenance gene seems therefore unlikely.
Key words
Yarrowia lipolytica Viruslike particles Curing UV lightAbbreviations
- UV
ultraviolet
- dsRNA
double-stranded RNA
- VLPs
viruslike particles, A and B alleles of the mating type locus
- TCA
trichloracetic acid
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