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Current Genetics

, Volume 8, Issue 2, pp 127–134 | Cite as

Ethidium bromide rejuvenation of senescent cultures of Podospora anserina: Loss of senescence-specific DNA and recovery of normal mitochondrial DNA

  • France Koll
  • Odile Begel
  • Anne-Marie Keller
  • Corinne Vierny
  • Léon Belcour
Article

Summary

The effect of ethidium bromide (EB) which is known to be able to “rejuvenate” senescent mycelia in Podospora anserina, has been investigated at the level of the mitochondrial DNA (mtDNA) by restriction analysis and molecular hybridization. While senescent mycelia display a very low growth ability and gross mtDNA modifications (tandem amplification of short sequences and disorganization of the mitochondrial chromosome: deletion of large sequences), the rejuvenated mycelia display a normal life span and contain a mtDNA in all respects identical to that of wild type mycelium (neither circular molecules nor amplified fragments could be detected). These results demonstrate a strict correlation between the senescent state and the presence of amplified mtDNA and suggest that EB rejuvenation could proceed by an efficient selection of intact mitochondrial chromosomes still present in senescent cultures.

Key words

Senescence Podospora Mitochondrial DNA Ethidium Bromide 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • France Koll
    • 1
  • Odile Begel
    • 1
  • Anne-Marie Keller
    • 1
  • Corinne Vierny
    • 1
  • Léon Belcour
    • 1
  1. 1.Centre de Génétique MoléculaireCentre National de la Recherche ScientifiqueGif-sur-YvetteFrance

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