Identification of regulatory genes of riboflavin permease and α-glucosidase in the yeast Pichia guilliermondii
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The method for a positive selection of Pichia guilliermondii yeast mutants which constitutively synthesize riboflavin (RF) permease has been developed. A genetic analysis revealed two regulatory genes of negative action (RFP80, RFP81) and one gene of positive action (RFP82); mutations in these loci determined the ability to synthesize RF permease in the medium without an inducer (α-glucosides). The constitutive mutants with cold-sensitive products of RFP80 and RFP81 genes were isolated. Interafelic complementation within RFP80 locus as well as restoration of the wild (inducible) phenotype in some hybrids between recessive rfp80 mutants and dominant RFP82c mutants were observed. These data suggest a protein structure of products of identified regulatory loci and a direct interaction of the products of RFP80 and RFP82 genes.
A meiotic segregants unable to synthesize RF permease in the inducer-containing media (genotype rfp82) were isolated by means of intragenic recombination in RFP82 locus. Epistasis-hypostasis test showed that gene RFP82 acted after gene RFP80. RFP80, RFP81 and RFP82 loci are involved in regulation of biosynthesis of both RF permease and α-glucosidase. The model for action of RFP80 and RFP82 gene products in the expression of RF permease and α-glucosidase structural genes of P. gulliermondii is presented.
Key wordsPichia guilliermondii Regulatory mutants Riboflavin transport α-Glucosidase
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- Inge-Vechtomov SG, Soidla TR (1978) In: Glotov NV, Poroshenko GG (eds) General genetics, vol 3. Evolutional and populational genetics (in Russian). ltogi nauki i tekhniki. VINITI AN SSSR, Moscow, pp 7–37Google Scholar
- Kozhin SA, Ter-Avanesian MD (1979) Issledovaniya po genetike (USSR) No. 8, pp 89–109Google Scholar
- Oshima Y (1982) In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 159–180Google Scholar
- Shavlovsky GM, Sibirny AA, Kshanovskaya BV, Koltun LV, Logvinenko EM (1979) Genetika (USSR) 15:1561–1568Google Scholar
- Sibirny AA, Ksheminskaya GP, Orlovskaya AG, Shavlovsky GM (1981) Biokhimiya (USSR) 46:1761–1763Google Scholar
- Sibirny AA, Shavlovsky GM, Goloshchapova GV (1977a) Genetika (USSR) 13:872–879Google Scholar
- Sibirny AA, Shavlovsky GM, Kshanovskaya BV, Naumov GI (1977b) Genetika (USSR) 13:314–321Google Scholar
- Sibirny AA, Shavlovsky GM, Ksheminskaya GP, Orlovskaya AG (1977c) Mikrobiologiya (USSR) 46:376–378Google Scholar
- Sibirny AA, Shavlovsky GM, Ksheminskaya GP, Orlovskaya AG (1977d) Biokhimiya (USSR) 42:1841–1851Google Scholar
- Sibirny AA, Shavlovsky GM, Ksheminskaya GP, Orlovskaya AG (1978) Biokhimiya (USSR) 43:1414–1422Google Scholar
- Sibirny AA, Shavlovsky GM, Ksheminskaya GP, Orlovskaya AG (1979) Biokhimiya (USSR) 44:1558–1568Google Scholar
- Sibirny AA, Zharova VP, Kshanovskaya BV, Shavlovsky GM (1977e) Tsitologiya i genetika (USSR) 11:330–333Google Scholar
- Wiame J-M (1973) In: Suomalainen H, Waller C (eds) Proc 3d Intern Spec Symp on Yeasts, part 11. Print Oy, Otaniemi-Helsinki, pp 307–330Google Scholar