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Current Genetics

, Volume 8, Issue 2, pp 147–154 | Cite as

Isolation, characterization, phosphorylation and site of synthesis of Spinacia chloroplast ribosomal proteins

  • Mark Posno
  • Marjolein van Noort
  • Roger Débise
  • Gert S. P. Groot
Article

Summary

We have characterized the ribosomal proteins from Spinacia chloroplasts using two-dimensional gel electrophoresis. The 30S and 50S subunits contain 23–25 and 36 ribosomal proteins, respectively. In contrast to prokaryotic ribosomes, chloroplast ribosomes contain at least one (and possibly two) phosphorylated ribosomal proteins. Isolated chloroplasts synthesize in the presence of (35S) labeled methionine and cysteine at least seven 30S and thirteen 50S ribosomal proteins which are assembled into (pre)ribosomes. This suggests that about one third of the chloroplast ribosomal proteins is encoded by the chloroplast DNA itself. The identity of several labeled proteins in the two-dimensional gel electrophoretic patterns which did not comigrate with stained chloroplast ribosomal proteins is discussed.

Key words

Chloroplast Ribosomal proteins Spinacia oleracea Phosphorylation Protein synthesis 

Abbreviations

CBB

Coomassie Brilliant Blue

CHI

cycloheximide

cp

chloroplast

DTT

dithiotreitol

EDTA

ethylene diamine tetraacetate

EGTA

ethylene glycol-bis (β-amino ethyl ether) N,N′-tetraacetic acid

kD

kilodalton

LHCP

light harvesting chlorophyll a/b protein

PMSF

phenyl methyl sulfonyl fluoride

RuBPCase

ribulose-1,5-bisphosphate carboxylase

SDS

sodiumdodecylsulphate

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Mark Posno
    • 1
  • Marjolein van Noort
    • 1
  • Roger Débise
    • 1
  • Gert S. P. Groot
    • 1
  1. 1.Biochemical LaboratoryFree UniversityAmsterdamThe Netherlands

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