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Current Genetics

, Volume 14, Issue 4, pp 381–385 | Cite as

Molecular cloning of the ARG7 gene of Schizosaccharomyces pombe encoding argininosuccinate lyase

  • Jacques E. Remacle
  • Didier Breyer
  • Roland Loppes
Article

Summary

A gene bank of Sau3A partially restricted Schizosaccharomyces pombe DNA in YEp13 was used to transform an arg4 mutant of Saccharomyces cerevisiae. One colony was recovered which contained the YEp13 plasmid bearing a large insert complementing the argininosuccinate lyase (ASL) mutation. As shown by restriction mapping and subcloning experiments, the DNA sequence required for complementation is localized on a 2 kb BamHI-BamHI fragment. The plasmid complemented several S. cerevisiae arg4 mutants of independent origin and a S. pombe arg7 mutant lacking ASL. Low but significant ASL activities were detected in crude extracts of these transformants. No complementation of the E. coli argH mutant was observed. Southern blot hybridizations showed that the insert originates from the S. pombe genome. No cross-hybridization was found between this sequence and S. cerevisiae DNA. It can be concluded that the cloned DNA fragment bears the S. pombe ARG7 gene coding for ASL.

Key words

Schizosaccharomyces pombe Saccharomyces cerevisiae Argininosuccinate lyase Gene cloning 

Abbreviations

ASL

argininosuccinate lyase

bp

base pair

kb

kilobase pair

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Jacques E. Remacle
    • 1
  • Didier Breyer
    • 1
  • Roland Loppes
    • 1
  1. 1.Laboratory of Molecular Genetics, Department of BotanyUniversity of LieǵeLiégeBelgium

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