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Current Genetics

, Volume 14, Issue 4, pp 375–379 | Cite as

The primary structure of the leul + gene of Schizosaccharomyces pombe

  • Yoshiko Kikuchi
  • Yasue Kitazawa
  • Hiroyuki Shimatake
  • Masayuki Yamamoto
Original Articles

Summary

A DNA fragment which carries the leul gene encoding beta-isopropylmalate dehydrogenase in Schizosaccharomyces pombe has been isolated by complementation of an E. coli leuB mutation. This 1.5 kb DNA fragment complements not only the S. pombe leul mutation, but also the S. cerevisiae leu2 mutation. The nucleotide sequence of the essential part of the leul gene and its flanking regions was determined. This sequence contains an open reading frame of 371 codons, from which a protein having a Mr = 39,732 can be predicted. The deduced amino acid sequence and its codon usage were compared with those of the S. cerevisiae LEU2 protein. The cloned DNA will be a useful marker when transforming S pombe.

Key words

Schizosaccharomyces pombe leul Cloning DNA sequence 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Yoshiko Kikuchi
    • 1
  • Yasue Kitazawa
    • 1
  • Hiroyuki Shimatake
    • 1
  • Masayuki Yamamoto
    • 2
  1. 1.Department of Molecular BiologyToho University, School of MedicineTokyoJapan
  2. 2.Laboratory of Molecular Genetics, Institute of Medical ScienceUniversity of TokyoTokyoJapan

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