Virus-like particles from the yeast Yarrowia lipolytica
Article
Received:
- 51 Downloads
- 8 Citations
Summary
Four out of the 24 strains of the yeast Yarrowia lipolytica we have checked for the presence of virus-like particles (VLPs) proved to contain encapsidated double-stranded RNA (dsRNA) molecules, 4.9 kb long. A major VLP polypeptide of MW 80,000 was observed in all 4 cases, and a second one of MW 77,000 in three cases. dsRNA from the VLPs harboring only the larger polypeptide showed little homology with the 3 others. We have found no homology between VLP dsRNAs and host DNA or dsRNAs from Saccharomyces cerevisiae, and no relationship between the presence of VLPs and a possible killer phenomenon in Y. lipolytica.
Key words
Virus-like particles Double-stranded RNA Yeast Yarrowia lipolyticaPreview
Unable to display preview. Download preview PDF.
References
- Adler J, Mackenzie DWR (1972) Abstr Ann Meet Soc Microbiol 8Google Scholar
- Bostian KA, Burn VE, Jayachandran S, Tipper DJ (1983) Nucleic Acids Res 11:1077–1097Google Scholar
- Buck KW (1980) Viruses and killer factors in fungi. In: Gooday GW, Lloyd D, Trinci APJ (eds) The Eukaryotic Microbial Cell. University Press, Cambridge, pp 329–375Google Scholar
- Fairbanks G, Steck TL, Wallach DFH (1971) Biochemistry 10:2606–2617Google Scholar
- Field LJ, Bobek LA, Brennan VE, Reilly JD, Bruenn JA (1982) Cell 31:193–200Google Scholar
- Franklin RM (1966) Proc Natl Acad Sci USA 55:1504–1511Google Scholar
- Groves DP, Clare JJ, Oliver SG (1983) Current Genetics 7:185–190Google Scholar
- Hastie ND, Brennan V, Bruenn JA (1978) J Virol 28:1002–1005Google Scholar
- Herring AJ, Bevan EA (1974) J Gen Virol 22:387–394Google Scholar
- Kirby KS (1968) Isolation of nucleic acids with phenolic solvents. In: Grossman L, Moldave K (eds) Methods in Enzymology, XII part B Nucleic Acids. Academic Press, New York, pp 87–88Google Scholar
- Kozlova TM (1973) Mikrobiologiya 42:745–747Google Scholar
- Laemmli UK (1970) Nature 227:680–685Google Scholar
- Landgridge R, Gomatos PJ (1963) Science 141:694–698Google Scholar
- Lemke PA (1979) Viruses and plasmids in fungi. Marcel Dekker, New YorkGoogle Scholar
- Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor, New YorkGoogle Scholar
- Mehta RJ, Nash CH III, Bozzola JJ (1982) J Bacteriol 152:502–505Google Scholar
- Mitchell I de G, Bèvan EA (1973) Genet Res Camb 22:169–179Google Scholar
- Mitchell I de G, Bevan EA (1974) Genet Res Camb 23:37–45Google Scholar
- Nesterova GF, Karner J, Soom YO (1973) Mikrobiologiya 42:162–165Google Scholar
- Nesterova GF, Karner J, Scorn YO, Kozlova TM (1978) Z Allg Mikrobiol 18:203–208Google Scholar
- Oliver SG, McCready SJ, Holm C, Sutherland PA, McLaughlin CS, Cox BS (1977) J Bacteriol 130:1303–1309Google Scholar
- Revet B, Delain E (1982) Virology 123:29–44Google Scholar
- Royer HD, Hollenberg CP (1977) Molec Gen Genet 150:271–284Google Scholar
- Shalitin C, Fisher I (1975) Biochim Biophys Acta 414:263–272Google Scholar
- Thomas PS (1980) Proc Natl Acad Sci USA 77:5201–5205Google Scholar
- Tipper DJ, Bostian KA (1984) Microbiol Rev 48:125–156Google Scholar
- Vodkin M (1977) J Virol 21:516–521Google Scholar
- Volkoff O, Walters T (1970) Can J Genet Cytol 12:621–626Google Scholar
- Vogt VM (1980) Purification and properties of S1 nuclease from Aspergillus. In: Grossman L, Moldave K (eds) Methods in Enzymology, 65 part I Nucleic Acids. Academic Press, New York, pp 248–255Google Scholar
- Welsh JD, Leibowitz MJ (1982) Proc Natl Acad Sci USA 79:786–789Google Scholar
- Wickner RB (1983a) Arch Biochem Biophys 222:1–11Google Scholar
- Wickner RB (1983b) Molec Cell Biol 3:654–661Google Scholar
- Wickner RB, Leibowitz MJ (1977) Genetics 87:453–469Google Scholar
- Zamir A, Maina C, Fink GR, Szalay AA (1981) Proc Natl Acad Sci USA 78:3496–3500Google Scholar
Copyright information
© Springer-Verlag 1985