Current Genetics

, Volume 10, Issue 6, pp 443–447

Genetic mapping of eleven spo genes essential for ascospore formation in the fission yeast Schizosaccharomyces pombe

  • Masao Kishida
  • Chikashi Shimoda
Original Articles

DOI: 10.1007/BF00419871

Cite this article as:
Kishida, M. & Shimoda, C. Curr Genet (1986) 10: 443. doi:10.1007/BF00419871


Sporulation-deficient mutants of the fission yeast Schizosaccharomyces pombe were isolated from a homothallic strain mutagenized with ethyl methanesulfonate. Complementation tests defined two new genetic loci (spo19 and spo20) essential for ascospore formation, in addition to the 18 known spo loci (Bresch et al. 1968). A novel mapping procedure using random spore analysis prior to tetrad analysis allowed us to map 11 spo genes. Four genes (spo3, spo15, spo19 and spo20) were mapped on chromosome I, 6 genes (spo2, spo4, spoS, spo6, spo14 and spo18) on chromosome II and 1 gene (spo13) on chromosome III. Although there was no noticeable clustering of spo genes on the chromosomes, three pairs of linked genes (spo15-spo20, spo3-spo19 and spo2-spo18) were found.

Key words

Mapping Sporulation Yeast Schizosaccharomyces pombe 

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Masao Kishida
    • 1
  • Chikashi Shimoda
    • 1
  1. 1.Department of Biology, Faculty of ScienceOsaka City UniversitySumiyoshi-ku, OsakaJapan

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