Current Genetics

, Volume 8, Issue 4, pp 299–305 | Cite as

The mitochondrial plasmid of Podospora anserina: A mobile intron of a mitochondrial gene

  • H. D. Osíewacz
  • K. Esser
Article

Summary

In the ascomycete Podospora anserina strain ageing (senescence) is caused by a mitochondrial plasmid. In juvenile mycelia it is an integral part of the mtDNA and becomes liberated during ageing. The nucleotide sequence of this plasmid and of its flanking regions was determined. It consists of 2,539 by and contains an un identified reading frame (URF) originating in the adjacent mtDNA upstream of excision point 1. Within the URF a putative 48 by autonomously replicating sequence (ars) was identified. At both excision sites of the plasmid there are two short nonidentical interrupted palindromes and a few base pairs apart from these palindromes, both upstream and downstream, two short inverted repeats are localised. The experimental data make it evident that the mt plasmid is an intron of the cytochrome c oxidase gene (subunit I) which may be excised at the DNA level and thus become the mobile infective agent causing senescence. The concept of this mobile intron and current hypotheses concerning the relationship between introns and transposons are stressed.

Key words

Podospora anserina mt plasmid DNA sequence Mobile intron 

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References

  1. Bernardi G, Baldacci G, Bernardi G, Faugeron-Fonty G, Gaillard C, Goursot R, Huyard A, Mangin M, Marotta R, de Zamaroczy M (1980) In: Kroon AM, Saccone C (eds) The Organization and Expression of the Mitochondrial Genome. Elsevier/North-Holland Biomedical Press, Amsterdam New York Oxford, pp 21–32Google Scholar
  2. Bolivar F, Rodrigez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Corsa JH, Falkow S (1977) Gene 2:95–113Google Scholar
  3. Bonitz SG, Coruzzi G, Thalenfeld BE, Tzagoloff A (1980) J Biol Chem 255:11927–11941Google Scholar
  4. Borst P, Grivell LA (1981) Nature 289:439–440Google Scholar
  5. Burger G, Scriven C, Machleidt W, Werner S (1982) EMBO J 1:1385–1391Google Scholar
  6. Crews S, Ojala D, Posakony J, Nishiguchi J, Attardi G (1979) Nature 277:192–198Google Scholar
  7. Cummings DJ, Belcour L, Grandchamp C (1979) Mol Gen Genet 171:229–238Google Scholar
  8. de Jonge JC, de Vries H (1983) Curr Genet 7:21–28Google Scholar
  9. Esser K (1974) Podospora anserina. In: King RC (ed) Handbook of Genetics I. Plenum Press, New York, pp 531–551Google Scholar
  10. Esser K, Stahl U, Tudzynski P, Kuck U (1982) Patent EP 82,104,5 82.0Google Scholar
  11. Esser K (1984) In: The 1984 Sandoz Lectures in Gerontology. Academic Press, New York (in press)Google Scholar
  12. Gargouri A, Lazowska J, Slonimski P (1983) In: Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1983: Nucleomitochondrial interaction. De Gruyter, Berlin, pp 259–268Google Scholar
  13. Goursot R, Mangin M, Bernardi G (1982) EMBO J 1:705–711Google Scholar
  14. Grivell LA, Hensgens LAM, Osinga KA, Tabak HF, Boer PH, Crusius JBA, van der Laan JC, de Haan M, van der Horst G, Evers RF, Arnberg AC (1982) In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial Genes. Cold Spring Harbor Laboratory, pp 225–240Google Scholar
  15. Hudspeth MES, Ainley WM, Shumard WM, Butow RA (1982) Cell 30:617–626Google Scholar
  16. Kück U, Stahl U, Esser K (1981) Curr Genet 3:151–156Google Scholar
  17. Maxam A, Gilbert W (1980) Meth Enzymol 65:499–560Google Scholar
  18. Michel F, Dujon B (1983) EMBO J 2:33–38Google Scholar
  19. Michel F, Jacquier A, Dujon B (1982) Biochemie 64:867–881Google Scholar
  20. Nobrega FG, Tzagoloff A (1980) J Biol Chem 255:9828–9837Google Scholar
  21. Osiewacz HD, Esser K (1983) Curr Genet 7:219–223Google Scholar
  22. Stahl U, Kück U, Tudzynski P, Esser K (1980) Mol Gen Genet 178:639–646Google Scholar
  23. Stahl U, Lemke PA, Tudzynski P, Kück U, Esser K (1978) Mol Gen Genet 162:341–343Google Scholar
  24. Stahl U, Tudzynski P, Kück U, Esser K (1982) Proc Natl Acad Sci USA 79:3641–3645Google Scholar
  25. Tudzynski P, Stahl U, Esser K (1980) Curr Genet 2:181–184Google Scholar
  26. Tudzynski P, Stahl U, Esser K (1982) Curr Genet 6:219–222Google Scholar
  27. Wright RM, Cummings DJ (1983a) Curr Genet 7:151–157Google Scholar
  28. Wright RM, Cummings DJ (1983b) Nature 301:86–88Google Scholar
  29. Wright RM, Horrum MA, Cummings DJ (1982) Cell 29:505–515Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • H. D. Osíewacz
    • 1
  • K. Esser
    • 1
  1. 1.Lehrstuhl für Allgemeine BotanikRuhr-UniversitätBochum 1Germany

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