Current Genetics

, Volume 3, Issue 1, pp 13–21 | Cite as

Mitochondrial DNA amplification in senescent cultures of Podospora anserina: Variability between the retained, amplified sequences

  • Léon Belcour
  • Odile Begel
  • Marie-Odile Mossé
  • Corinne Vierny
Original Articles

Summary

The non-nuclear DNA of a number of independent senescent cultures of Podospora anserina was extracted and studied. In all cases, a specific repetitive DNA (SEN-DNA) arranged in multimeric sets of circular molecules, was identified. Depending on the senescent culture, the SEN-DNA was found either in a band of about same density as the mitochondrial DNA from young mycelia (1.694 g/cm3) or in a band of higher density (1.699 g/cm3). Electron microscopy, restriction enzyme analysis and Southern hybridization experiments allowed us to establish that: (1) SEN-DNAs obtained from independent senescent cultures, both from the same strain and from different strains, can differ in the size of their monomer unit (from 2.5 to 6.3 kb). (2) All SEN-DNAs hybridize with mitochondrial DNA of a young culture and not with nuclear DNA. (3) These SEN-DNAs belong to two classes which hybridize with two non-overlapping regions of the mitochondrial chromosome.

Key words

Senescence Podospora Mitochondrial DNA DNA Amplification 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Léon Belcour
    • 1
  • Odile Begel
    • 1
  • Marie-Odile Mossé
    • 1
  • Corinne Vierny
    • 1
  1. 1.Centre de Génétique Moléculaire, Centre National de la Recherche ScientifiqueGif-sur-YvetteFrance

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