Current Genetics

, Volume 12, Issue 7, pp 503–509 | Cite as

Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number

  • Hans Koff
  • Cornelia Schmidt
  • Gerlinde Wiesenberger
  • Carlo Schmelzer
Original Articles

Summary

A gene bank of a yeast wild type DNA in the high copy number vector YEp 13 was screened for recombinant plasmids which suppress the mitochondrial RNA splice defect exerted by mutant M1301, a −1 by deletion in the first intron of the mitochondrial COB gene (bIl). A total of 17 recombinant plasmids with similar suppressor activity were found. Restriction mapping and cross-hybridization of the inserts revealed that these 17 plasmids contain three different inserts, all lacking any extended sequence homology. Each of the inserts, when present in high copy number, has a similar suppressor activity: high in the presence of mutation M1301 in bll, a group II intron, and low but significant with the presence of few mutants in bI2 and bI3 of the COB gene, both of which are group I introns.

Key words

Suppression by high copy number RNA splicing Yeast mitochondria 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Hans Koff
    • 1
  • Cornelia Schmidt
    • 1
  • Gerlinde Wiesenberger
    • 1
  • Carlo Schmelzer
    • 1
  1. 1.Institut für Genetik und MikrobiologieUniversität MünchenMünchen 19Germany

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