Archives of Microbiology

, Volume 135, Issue 1, pp 63–67 | Cite as

Nucleotide pools of growing, synchronized and stressed cultures of Saccharomyces cerevisiae

  • Günther Ditzelmüller
  • Wilfried Wöhrer
  • Christian P. Kubicek
  • Max Röhr
Original Papers

Abstract

High pressure liquidd chromatography has been used to study the acid soluble nucleotide pool of Saccharomyces cerevisiae under different conditions of growth. ATP, ADP, AMP, NAD, GTP, UTP, UDP, CTP, CDP, and UDP-sugars plus UMP could be separated and were found in concentrations higher than 0.1 μmol per g yeast cell dry weight (=detection limit). During glucose-limited continuous culture the levels of individual nucleotides depended on the growth rate, which was most pronounced with pyrimidine (uridine, cytidine) nucleotides. The energy charge (E.C.) remained high (0.9) at all growth rates (0.07–0.3 h-1). During synchronized growth at a constant growth rate (0.11 h-1) almost all nucleotide levels and the E.C. remained at constant values with the only exception of UDP-sugars and UMP of which increased levels were found during the phase of budding. Under conditions of metabolic stress (addition of antimycin A, deoxyglucose plus iodoacetate) pronounced changes in the levels of purine (adenine and guanine) nucleotides and the E.C. were observed. All other nucleotides were less influenced by these conditions. Only under these conditions IMP accumulation was observed. The results strongly argue against the significance of purine nucleotide or E.C. measurements under viable conditions. In contrast, changes in the levels of pyrimidine nucleotides seem to be indicative of changes in the flux through the metabolic pathways where they act as coenzymes.

Key words

Nucleotide pools Continuous cultivation Synchronized growth Saccharomyces cerevisiae 

Abbreviation

HPLC

High pressure liquid chromatography

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Günther Ditzelmüller
    • 1
  • Wilfried Wöhrer
    • 1
  • Christian P. Kubicek
    • 1
  • Max Röhr
    • 1
  1. 1.Institut für Biochemische Technologie und MikrobiologieTechnische Universität WienWienAustria

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