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Archives of Microbiology

, Volume 135, Issue 1, pp 36–41 | Cite as

Energy metabolism and biosynthesis of Vibrio succinogenes growing with nitrate or nitrite as terminal electron acceptor

  • Martin Bokranz
  • Joachim Katz
  • Imke Schröder
  • Anthony M. Roberton
  • Achim Kröger
Original Papers

Abstract

  1. 1.
    Growth of Vibrio succinogenes with nitrate as terminal electron acceptor was found to be a function of the following two catabolic reactions:
    $$HCO _2^ - + NO _3^ - + H^ + \to CO_2 + NO _2^ - + H_2 O$$
    (a)
    $$3HCO _2^ - + NO _2^ - + 5H^ + \to 3CO_2 + NH _4^ + + 2H_2 O.$$
    (b)

    The latter reaction (b) was responsible for growth with nitrite.

     
  2. 2.

    Either succinate or fumarate could serve as sole carbon source during growth with nitrate or nitrite. Biosynthesis from succinate proceeded via fumarate. The ATP requirement for cell synthesis from succinate was equal to that calculated earlier for growth with fumarate as carbon source and electron acceptor (Brounder et al. 1982).

     
  3. 3.

    The cell yield at infinite dilution rate (Ymax) as obtained with chemostat cultures was 8.5g dry cells/mol formate with either nitrate or nitrite as acceptor. This value amounts to 60% of that measured earlier with fumarate as acceptor (Mell et al. 1982).

     
  4. 4.

    Membrane vesicles prepared from V. succinogenes catalyzed electron transport from H2 to nitrate. The reaction was dependent on the menaquinone present in the membrane.

     
  5. 5.

    Electron transport with H2 and nitrite was coupled to the phosphorylation of ADP. The P/H2 ratio with nitrite was 40% of that measured with fumarate as acceptor using the same preparation. The phosphorylation but not the electron transport was abolished by an uncoupling agent.

     

Key words

Phosphorylative nitrite reduction Nitrate reduction Vibrio succinogenes 

Abbreviations

TRIS

tris (hydroxymethyl)aminomethane

MOPS

3-(N-morpholino)propanesulfonate

MK

menaquinone

FCCP

carbonylcyanide-4-trifluoromethoxyphenylhydrazone

EDTA

ethylene diamine tetraacetate

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Martin Bokranz
    • 1
  • Joachim Katz
    • 1
  • Imke Schröder
    • 1
  • Anthony M. Roberton
    • 1
  • Achim Kröger
    • 1
  1. 1.Fachbereich Biologie, MikrobiologiePhilipps-UniversitätMarburgFederal Republic of Germany

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