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On characterization of the growth of Escherichia coli in batch culture

Abstract

Several growth monitoring parameters, including adenine nucleotide contents, were measured during Escherichia coli K12 batch cultivation in mineral medium with glucose. The adenylate energy charge with its mean value of 0.83 remained roughly stable during growth. The total adenylate and ATP pools (nmol/mg dry weight), and also the individual cell volume changed with a pattern of two maxima at approximately 4 and 10 h of cultivation. After the exhaustion of the glucose from the growth medium the adenylate energy charge, the pools of ATP and total adenylate started to decrease marking the onset of the stationary growth phase. Our data indicate that actually there was only a limited period within the logarithmic growth phase during which the growth might have been balanced: during this period of 1.5 h different growth monitoring parameters (optical absorbance, cell number, total cell volume, and ATP content per ml) increased with almost equal rates. Moreover, as ATP pool and median cell volume during this short period were approximately constant, the culture might have been even in the steady state.

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References

  1. Atkinson DE (1968) The energy charge of the adenylate pool as a regulatory parameter. Interaction with feed-back modifiers. Biochemistry 7:4030–4034

  2. Bergmeyer HU, Bernt E, Schmidt F, Stork H (1974) d-Glucose determination with hexokinase and glucose-6-phosphate dehydrogenase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 3. Academic Press Inc, New York, pp 1196–1201

  3. Bossuyt R (1978) Usefulness of an ATP assay technique in evaluating the somatic cell content of milk. Milchwissenschaft 33:11–13

  4. Campbell A (1957) Synchronization of cell division. Bacteriol Rev 21:263–272

  5. Cole HA, Wimpenny JWT, Hughes DE (1967) The ATP pool in Escherichia coli. I. Measurement of the pool using a modified luciferase assay. Biochim Biophys Acta 143:445–453

  6. Donohue TJ, Bernlohr RW (1978) Effect of cultural conditions on the concentrations of metabolic intermediates during growth and sporulation of Bacillus licheniformis. J Bacteriol 135:363–372

  7. Fynn GH, Davison JA (1976) Adenine nucleotide pool and energy charge of a thyrothricin-producing strain of Bacillus brevis. J Gen Microbiol 94:68–74

  8. Hennen PE, Carter HB, Nunn WD (1978) Changes in macromolecular synthesis and nucleoside triphosphate levels during glycerol-induced growth stasis of Escherichia coli. J Bacteriol 136:929–935

  9. Holms WH, Hamilton JD, Robertson AG (1972) The rate of turnover of the adenine triphosphate pool of Escherichia coli growing aerobically in simple defined media. Arch Mikrobiol 83:95–109

  10. Kahru A, Liiders M, Vanatalu K, Vilu R (1982) Adenylate energy charge during batch culture of Thermoactinomyces vulgaris 42. Arch Microbiol 133:142–144

  11. Kubitschek HB (1958) Electronic counting and sizing of bacteria. Nature 182:234–235

  12. Markland FS, Wadkins CL (1966) Adenosine triphosphate-adenosine 5′-monophosphate phosphotransferase of bovine liver mitochondria. II. General kinetics and structural properties. J Biol Chem 241:4136–4145

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Correspondence to Anne Kahru.

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Kahru, A., Vilu, R. On characterization of the growth of Escherichia coli in batch culture. Arch. Microbiol. 135, 12–15 (1983). https://doi.org/10.1007/BF00419475

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Key words

  • Escherichia coli K12
  • Batch culture
  • Growth monitoring
  • Adenylate content
  • Balanced growth