Antonie van Leeuwenhoek

, Volume 54, Issue 2, pp 149–163

Isolation and characterisation of dog uropathogenic Escherichia coli strains and their fimbriae

  • E. Garcia
  • H. E. N. Bergmans
  • J. F. van den Bosch
  • I. Ørskov
  • B. A. M. van der Zeijst
  • W. Gaastra


A number of Escherichia coli strains have been isolated from dogs with urinary tract infections. These strains have been characterised with respect to their O, K, H, and fimbrial antigens, colicin production, antibiotic resistance, plasmid content and their ability to haemagglutinate erythrocytes from various species. Crossed immunoelectrophoresis of fimbrial extracts, as well as the reaction of partly purified fimbriae of a number of these strains with monoclonal antibodies revealed homology or a strong crossereaction with an F12 fimbrial subunit protein of human uropathogenic E. coli strains. Unlike human F12 fimbriae producing strains, the dog isolates did agglutinate dog erythrocytes in the presence of D-mannose but not human erythrocytes, indicating that the adhesin carried by these strains is different from the adhesin on fimbriae of human uropathogenic E. coli. Similar indications were obtained from experiments with latex beads coated with the receptor for P-fimbriae. These beads were agglutinated by Escherichia coli strains from human urinary tract infections, but not by the dog isolates described here. Preliminary adhesion experiments of human and dog Escherichia coli to human bladder epithelial and canine kidney epithelial cells also showed differences in adhesion depending on the origin of the strain tested.

Key words

Escherichia coli urinary tract infection dogs fimbriae 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baselier, P.J.A.M. (1983) Acute bacteriele urineweg-infecties in de huisartspraktijk. PhD. thesis. Katholieke Universiteit Nijmegen, The NetherlandsGoogle Scholar
  2. Birnboim, M.C. & F.G. Doly (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7: 1513–1523Google Scholar
  3. Carbonetti, N.H. & P.H. Williams (1984) A cluster of five genes specifying the aerobactin iron uptake system of plasmid Col V-K30. Infect. Immun. 45: 7–12Google Scholar
  4. De Ree, J.M., P. Schwillens & J.F. van den Bosch (1985) Monoclonal antibodies that recognize the P-fimbriae F71, F72, F9 and F11 from uropathogenic E. coli. Infect. Immun. 50: 900–904Google Scholar
  5. De Ree, J.M., P. Schwillens & J.F. van den Bosch (1986) Monoclonal antibodies for serotyping the P-fimbriae of uropathogenic E. coli. J. of Clin. Microbiol. 24: 121–125Google Scholar
  6. De Ree, J.M. (1986) Molecular and serological characterization of P-fimbriae from uropathogenic Escherichia coli. PhD. thesis, University of Limburg, Maastricht, The NetherlandsGoogle Scholar
  7. Hacker, J., G. Smidt, C. Hughes, S. Knapp, M. Marget & W. Goebel (1985) Cloning and characterization of genes involved in production of mannose — resistant, neurammidase —susceptible (X) fimbriae from a uropathogenic 06:K15:H31 Escherichia coli strain. Infect. Immun. 47: 434–440Google Scholar
  8. Hagberg, I., U. Jodal, T.K. Korhonen, G. Lidin-Janson, U. Lindberg & C. Svanborg-Edén (1981) Adhesion, haemagglutination and virulence of E. coli causing urinary tract infection. Infect. Immun. 31: 564–570Google Scholar
  9. Hughes, C., J. Hacker, A. Roberts & W. Goebel (1983) Haemolysin production as a virulence marker in urinary tract infections caused by E. coli. Infect. Immun. 39: 545–551Google Scholar
  10. Kaijser, B., L.A. Hanson, U. Jodal, G. Lidin-Janson & J.B. Robbins (1977) Frequency of E. coli K antigens in urinary tract infections in children. Lancet i: 663–664Google Scholar
  11. Källenius, G., R. Möllby, S.B. Svenson, J. Winberg, A. Lundblad, S. Svensson & B. Cedergren (1980) The pk antigen as receptor for the haemagglutinin of pyelonephritic Escherichia coli FEMS Microbiol. Letters 7: 297–302Google Scholar
  12. Kivistó, A-K, H. Vasenius, L-A. Lindberg & M. Sandholm (1978) Desorption assay. A functional in vitro test for measuring the ahesion of E. coli on the urinary tract epíthelium. Invest. Urol. 15: 412–415Google Scholar
  13. Klemm, P., I. Ørskov & F. Ørskov (1982) F7 and type 1 like fimbriae from three Escherichia coli strains isolated from urinary tract infections; protein chemical and immunological aspects. Infect. Immun. 36: 462–468Google Scholar
  14. Korhonen, T.K., V. Väisänen, P. Kallio, E-L. Nurmiaho-Lassila, H. Ranta, A. Siitonen, J. Elo, S.B. Svenson & C. Svanborg-Edén (1982) The role of pili in the adhesion of Escherichia coli to human urinary tract epithelial cells. Scand. J. Infect. Dis. 33 (suppl.): 26–33Google Scholar
  15. Korhonen, T.K., V. Väisänen-Rhen, M. Rhen, A. Porc, J. Parkkinen & J. Finne (1984) Escherichia coli fimbriae recognizing sialyl galactosides. J. Bacteriol. 159: 762–766Google Scholar
  16. Krone, W.J.A., P. de Vries, G. Koningstein, A.J.R. De Jonge, F.K. De Graaf & B. Oudega (1986) Uptake of cloacin DF13 by susceptible cells: removal of immunity protein and fragmentation of cloacin molecules. J. Bacteriol. 166: 260–268Google Scholar
  17. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680–685Google Scholar
  18. Lindberg, F., B. Lund, L. Johansson & S. Normark (1987) Localization of the receptor-binding protein adhesin at the tip of the bacterial pilus. Nature 328: 84–87Google Scholar
  19. Lund, B. (1987) The molecular mechanism of uroepithelial binding by uropathogenic Escherichia coli; variation of adhesion specificity. PhD. thesis. University of Umeä. Umeä, SwedenGoogle Scholar
  20. Maniatis, T., E.F. Fritsch & J. Sambrook (1982) In: Molecular Cloning, A Laboratory Manual. Cold Spring Harbor LaboratoryGoogle Scholar
  21. Minshew, B., J. Jorgensen, G.W. Counts & S. Falkow (1978) Association of hemolysin production, hemagglutination of human erythrocytes and virulence of chiken embryos of extraintestinal Escherichia coli isolates. Infect. Immun. 28: 359–367Google Scholar
  22. Normark, S., M. Bäga, M. Göransson, F. Lindberg, B. Lund, M. Norgren & B-E. Uhlin (1985) Genetic analysis of bacterial pilus adhesins. In: T.K. Korhonen, E.A. Dawes & P.H. Mäkela (Eds) Enterobacterial Surface Antigens: Methods for Molecular Characterisation (pp. 39–52). Elsevier Science Publishers, Amsterdam, The NetherlandsGoogle Scholar
  23. Ørskov, I., F. Ørskov, A. Birch-Andersen, M. Kanamori & C. Svanborg-Edén (1982) O, K, H and fimbrial antigens in E. coli serotypes associated with pyelonephritis and cystitis. Scand. J. Infect. Dis. (suppl.) 33: 18–25Google Scholar
  24. Ørskov, I. & F. Ørskov (1983) The serology of E. coli fimbriae. Progress in Allergy 33: 80–105Google Scholar
  25. Ørskov, F. & I. Ørskov (1984) Serotyping of Escherichia coli. In: T. Bergan (Ed) Methods in Microbiology. Vol. 14 (pp. 43–112). Academic Press, LondonGoogle Scholar
  26. Ørskov, I. & F. Ørskov (1985) Escherichia coli in extra intestinal infections. J. Hyg. 95: 551–575Google Scholar
  27. Ørskov, I., A. Birch-Andersen, J. Duguid, J. Stenderup & F. Ørskov (1985) An adhesive protein capsule of Escherichia coli. Infect. Immun. 47: 191–200Google Scholar
  28. Oudega, B., P. Klaasen-Boor, G. Sneeuwloper & F.K. de Graaf (1977) Interaction of the complex between cloacin and its immunity protein and of cloacin with the outer and cytoplasmic membrane of sensitive cells. Eur. J. Biochem. 78: 445–453Google Scholar
  29. Rhen, M., P. Klemm, E. Wahlström, S.B. Svenson, G. Källenius & T.K. Korhonen (1983) P-fimbriae of Escherichia coli; immuno- and protein-chemical characterization of fimbriae from two pyelonephretic strains. FEMS Microbiol. Letters 18: 233–238Google Scholar
  30. Salit, I.E., J. Vavougios & T. Hofmann (1983) Isolation and characterization of Escherichia coli pili from diverse clinical scources. Infect. Immun. 42: 755–762Google Scholar
  31. Schmoll, T., J. Hacker & W. Goebel (1987) Nucleotide sequence of the sfaA gene coding for the S-fimbrial protein subunit of Escherichia coli. FEMS Microbiol. Letters 41: 229–235Google Scholar
  32. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517Google Scholar
  33. Svanborg-Edén, C., L.A. Hanson, U. Jodal, U. Lindberg & A. Sahl-Akerlund (1976) Variable adherence to normal human urinary epithelial cells of E. coli strains associated with various forms of urinary tract infections. Lancet i: 490–492Google Scholar
  34. Svanborg-Edén, C. & H.A. Hansson (1978) Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infect. Immun. 21: 229–237Google Scholar
  35. Svanborg-Edén, C., A. Fasth, L. Hagberg, L.A. Hanson, T. Korhonen & H. Leffler (1982) Host interaction with Escherichia coli in the urinary tract. In: L. Weinstein & B.N. Fields (Eds) Seminars in Infectious Desease. Vol. 4 (pp. 113–131). Bacterial vaccines. Thieme-Stratton, New YorkGoogle Scholar
  36. Väisänen, V., T.K. Korhonen, M. Jokinen, C.G. Gahmberg & C. Ehnholm (1982) Blood group M specific haemagglutinin in pyelonephritic Escherichia coli. Lancet i: 1192Google Scholar
  37. Väisänen-Rhen, V., J. Elo, E. Väisänen, A. Siitonen, I. Ørskov, F. Ørskov, S.B. Svenson, P.H. Mäkela & T.K. Korhonen (1984) P-fimbriated clones among uropathogenic E. coli strains. Infect. Immun. 43: 149–155Google Scholar
  38. Van den Bosch, J.F., U. Verboom-Sohmer, P. Postma, J. de Graaff & D.M. MacLaren (1980) Mannose sensitive and mannose resistant adherance to human uroepithelial cells and urinary virulence of Escherichia coli. Infect. Immun. 29: 226–233Google Scholar
  39. Van den Bosch, J.F., P. Postma, J. de Graaff & D.M. MacLaren (1981) Haemolysis by urinary Escherichia coli and virulence in mice. J. Med. Microbiol. 14: 321–331Google Scholar
  40. Van den Bosch, J.F., P. Postma, P.A.R. Koopman, J. de Graaff & D.M. MacLaren (1982) Virulence of urinary and faecal Escherichia coli in relation to serotype, haemolysis and haemagglutination. J. Hyg. 88: 567–577Google Scholar
  41. Van Die, I., G. Spierings, I. van Megen, E. Zuidweg, W. Hoekstra & H. Bergmans (1985) Cloning and genetic organization of the gene cluster encoding F71 fimbriae of a uropathogenic E. coli and comparison with the F72 gene cluster. FEMS Microbiol. Letters 28: 329–334Google Scholar
  42. Van Die, I., E. Zuidweg, W. Hoekstra & H.E.N. Bergmans (1986) The role of fimbriae of uropathogenic Escherichia coli as carrier of the adhesin involved in mannose-resistant haemagglutination. Microbial. Pathogenesis 1: 51–56Google Scholar
  43. Westerlund, B., A. Pere, T.K. Korhonen, A-K. Järvinen, A. Siitonen & P.H. Williams (1987) Characterisation of Escherichia coli associated with canine urinary tract infections. Res. Vet. Science 42: 404–406Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • E. Garcia
    • 1
  • H. E. N. Bergmans
    • 2
  • J. F. van den Bosch
    • 3
  • I. Ørskov
    • 4
  • B. A. M. van der Zeijst
    • 1
  • W. Gaastra
    • 1
  1. 1.Institute of Infectious Diseases and Immunology, Dept. of Bacteriology, Faculty of Veterinary MedicineUniversity of UtrechtUtrechtThe Netherlands
  2. 2.Dept. of Molecular Cell Biology, Transitorium 3University of UtrechtUtrechtThe Netherlands
  3. 3.Intervet International B.V.BoxmeerThe Netherlands
  4. 4.Statens SeruminstitutInternational Escherichia coli Centre (WHO)CopenhagenDenmark

Personalised recommendations