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Evaluation of an enzymatic immunoassay for free thyroxine determination in canine serum

Abstract

Free thyroxine (FT4) and cholesterol were measured in 400 dogs with either suspected hypothyroidism or dermatological signs such that hypothyroidism needed to be ruled out. Hypothyroidism was diagnosed in 68 dogs from the history, physical examination and stated lower reference limit (<7 pmol/L) for FT4 in euthryoid dogs. Dogs with FT4 concentrations in the range 6–9 pmol/L were finally categorized as hypo- or euthyroid either on the basis of retesting after 2 months or on their clinical response to thyroid replacement therapy over at least 2 months.

The enzyme immunoassay evaluated in this paper is considered to be of clinical value and offers many advantages compared with radioimmunoassays.

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Abbreviations

FT4:

free thyroxine fraction

MEIA:

microparticle enzyme immunoassay

RIA:

radioimmunoassays

T4:

thyroxine

TBG:

thyroxine-binding globulin

References

  1. Allain C.C., Poon L.S., Chan C.S.G., Richmond W. and Fu P.C., 1974. Enzymatic determination of total serum cholesterol. Clinical Chemistry, 20, 470–475

  2. Anderson R.R., Nixon D.A. and Akasha M.A., 1988. Total and free thyroxine and triiodothyronine in blood serum in animals. Comparative Biochemistry and Physiology, 89, 401–404

  3. Bigos S.T., Ridgway E.C., Kourides I.A. and Maloof F., 1978. Spectrum of pituitary alterations with mild and severe thyroid impairment. Journal of Clinical Endocrinology and Metabolism, 46, 317–325

  4. Bush B.M., 1991. The endocrine system: In: E.A. Chandler, D.J. Thompson, J.B. Sutton and C.J. Price (eds.), Canine Medicine and Therapeutics, 3rd edn., (Blackwell Scientific, Oxford), 329

  5. Chan D.W., Waud J.M., Hsu T.H., 1983. Clinical assessment of a radioimmunoassay for free thyroxine using a modified tracer. Journal of Nuclear Medicine, 24, 498–504

  6. Chastain C.B. and Ganjam V.K., 1986. Hypothyroidism and thyroditis. In: Clinical Endocrinology of Companion Animals, (Lea and Febiger, Philadelphia), 136

  7. Chattoraj S.C. and Watts N.B., 1986. Endocrinology. In: N.W. Tietz (ed.), Textbook of Clinical Chemistry, (W.B. Saunders, Philadelphia), 1119

  8. Evinger J.V., Nelson R.W. and Bottoms G.D., 1985. Thyrotropin-releasing hormone stimulation testing in healthy dogs. American Journal of Veterinary Research, 46, 1323–1325

  9. Ferguson D.C., 1984. Thyroid function tests in the dog. Recent concepts. Veterinary Clinics of North America: Small Animal Practice, 14, 783–808

  10. Ferguson D.C., 1988. The effect of non-thyroidal factors on thyroid function tests in dogs. Compendium on Continuing Education for the Practicing Veterinarian, 10, 1365–1377

  11. Furth E.D., Becker D.V., Nunez E.A. and Reid C.F., 1968. Thyroxine metabolism in the dog. Endocrinology, 82, 976–982

  12. Gosselin S.J., Capen C.C. and Martin S.L., 1981a. Histologic and ultrastructural evaluation of thyroid lesions associated with hypothyroidism in dogs. Veterinary Pathology, 18, 299–309

  13. Gosselin S.J., Capen C.C., Krakowka S. and Martin S.L., 1981b. Lymphocytic thyroiditis in dogs: induction with a local graft-versus-host reaction. American Journal of Veterinary Research, 42, 1856–1864

  14. Hillier A.P., 1975. The rate of triiodothyronine dissociation from binding sites and human plasma. Acta Endocrinologica, 80, 49–57

  15. Kaplan M.M., Larsen P.R., Crantz F.R., Dzau V.J., Rossing T.H. and Haddow J.E., 1982. Prevalence of abnormal thyroid function test results in patients with acute medical illnesses. American Journal of Medicine, 72, 9–16

  16. Kaptein, E.M., 1988. Thyroid hormone metabolism in nonthyroidal illness. In: Proceedings of the American College of Veterinary Internal Medicine, 6, 643–650

  17. Larsen P.R., 1982. Thyroid-pituitary interaction. Feedback regulation of thyrotropin secretion by thyroid hormones. New England Journal of Medicine, 306, 23–32

  18. Nelson R.W., Ihle S.L., Feldman E.C. and Bottoms G.D., 1991. Serum free thyroxine concentration in healthy dogs with hypothyroidism, and euthyroid dogs with concurrent illness. Journal of the American Veterinary Medical Association, 198, 1401–1407

  19. Oppenheimer J.H. and Surks M.I., 1964. Determination of free thyroxine in human serum: a theoretical and experimental analysis. Journal of Clinical Endocrinology and Metabolism, 24, 785–793

  20. Panciera D.L., 1990. Canine hypothyroidism. Part II. Compendium on Continuing Education for the Practicing Veterinarian, 12, 843–856

  21. Pardridge W.M., 1981. Transport of protein-bound hormones into tissues in vivo. Endocrine Review, 2, 103–123

  22. Peterson M.E. and Ferguson D.C., 1989. Thyroid diseases. In: S.J. Ettinger (ed.), Textbook of Internal Veterinary Medicine, 3rd edn., (W.B. Saunders, Philadelphia), 1641

  23. Refetoff S., Robin N.I. and Fang V.S., 1970. Parameters of thyroid function in serum of 16 selected vertebrate species: a study of PBI, serum T4, free T4, and the pattern of T4 and T3 binding to serum proteins. Endocrinology, 86, 793–805

  24. Reimers T.J., McCann J.P. and Cowan R.G., 1983. Effects of storage times and temperatures on T3, T4, LH, prolactin, insulin, cortisol and progesterone concentrations in blood samples from cows. Journal of Animal Science, 57, 683–691

  25. Robinson W.F., Shaw S.E., Stanley B. and Wyburn R.S., 1988. Congenital hypothyroidism in Scottish Deerhound puppies. Australian Veterinary Journal, 65, 386–389

  26. SAS Institute, 1989. SAS/STAT. SAS/VarComp Procedure, Users Guide, Version 6, 4th edn., (SAS Institute, Inc., Cary, NC)

  27. Solberg H.E., 1983. The theory of reference values. Part 5. Statistical treatment of collected reference values. Determination of reference limits. Journal of Clinical Chemistry and Clinical Biochemistry, 21, 749–760

  28. Vollset I. and Larsen H.J., 1987. Occurence of autoantibodies against thyroglobulin in Norwegian dogs. Acta Veterinaria Scandinavica, 28, 65–71

  29. Wang Y.S., Hershman J.M. and Pekary A.E., 1985. Improved ultrafiltration method for simultaneous measurement of free thyroxin and free triiodothyronine in serum. Clinical Chemistry, 31, 517–532

  30. Wartofsky L. and Burman K.D., 1982. Alterations in thyroid function in patients with systemic illness: the “euthyroid sick syndrome”. Endocrine Review, 3, 164–217

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Correspondence to S. I. Thoresen.

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Thoresen, S.I., Wergeland, R., Mørkrid, L. et al. Evaluation of an enzymatic immunoassay for free thyroxine determination in canine serum. Vet Res Commun 20, 411–420 (1996). https://doi.org/10.1007/BF00419178

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Keywords

  • dog
  • endocrinology
  • hormone
  • immunoassay
  • T4
  • thyroid
  • thyroxine