Current Genetics

, Volume 10, Issue 11, pp 857–869 | Cite as

The watermelon mitochondrial URF-1 gene: evidence for a complex structure

  • David B. Stern
  • Anne G. Bang
  • William F. Thompson
Original Articles


We have cloned and sequenced a fragment of watermelon mitochondrial DNA (mtDNA) which contains a gene homologous to mitochondrial URF-1 (Unidentified Reading Frame-1) of vertebrates, Drosophila yakuba and Aspergillus nidulans. URF-1 is thought to encode a component of the respiratory chain NADH dehydrogenase. Two coding regions in the watermelon gene are separated by approximately 1,450 by of untranslatable DNA. These two exons encode the central portions of URF-1, and are highly conserved. We postulate that three additional exons, selected by their map location and amino acid homology to other URF-1 sequences, encode the remainder of the polypeptide. This is the first description of a plant mitochondrial gene with multiple introns.

Key words

Intron Mitochondrial DNA Mitochondrial gene Unidentified reading frame 


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • David B. Stern
    • 1
  • Anne G. Bang
    • 1
  • William F. Thompson
    • 1
  1. 1.Department of Plant BiologyCarnegie Institution of WashingtonStanfordUSA

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