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Current Genetics

, Volume 10, Issue 1, pp 49–58 | Cite as

Integrative transformation of the yeast Yarrowia lipolytica

  • C. Gaillardin
  • A. M. Ribet
  • H. Heslot
Article

Summary

An EcoR1 shotgun of Yarrowia lipolytica DNA was inserted into the plasmid YIp333 which carries the LYS2 gene of S. cerevisiae. The resulting plasmid pool was transformed in both S. cerevisiae and Y. lipolytica. Whereas numerous replicating plasmids could be isolated from the S. cerevisiae Lys+ transformants, all transformants of Y. lipolytica so far analyzed were found to result from integrative transformation. This occurred at a frequency of 1 to 10 transformants per μg of input DNA. Co-transformation occurred at high frequency and resulted in tandem integration of 2 to 10 copies of the incoming DNA. Structural and segregational stability of the transforming DNA were both high.

Key words

Yarrowia lipolytica Heterologous gene expression Integrative transformation Homologous recombination 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • C. Gaillardin
    • 1
  • A. M. Ribet
    • 1
  • H. Heslot
    • 1
  1. 1.Laboratoire de GénétiqueInstitut National Agronomique Paris-GrignonParis Cedex 05France

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