Current Genetics

, Volume 10, Issue 5, pp 339–342

The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c

  • J. Verdière
  • F. Creusot
  • L. Guarente
  • P. P. Slonimski
Original Articles

Summary

The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differentially regulated by the product of CYP1. The HAP1 gene codes for a product which has previously been proved to be necessary for the expression of the heme dependent CYC1-UAS1 cis regulatory sequence. In this article, we show by complementation and recombination that CYP1 and HAP1 are the same gene, moreover we identify hap1-1 as an iso2-cytochrome c underproducer mutation of the CYP1 gene.

Key words

Saccharomyces cerevisiae Cytochrome c Regulatory gene 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Claisse M, Péré-Aubert G, Clavilier L, Slonimski PP (1970) Eur J Biochem 16:430–438Google Scholar
  2. Clavilier L, Péré G, Slonimski PP (1969) Mol Gen Genet 104:195–218Google Scholar
  3. Calvilier L, Péré-Aubert G, Somlo M, Slonimski PP (1976) Biochimie 58:155–172Google Scholar
  4. Guarente L, Mason T (1983) Cell 32:1279–1286Google Scholar
  5. Guarente L, Lalonde B, Gifford P, Alani E (1984) Cell 36:503–511Google Scholar
  6. Mortimer RK, Hawthorne DC (1966) Genetics 53:165–173Google Scholar
  7. Rothstein RJ, Sherman F (1980) Genetics 94:871–889Google Scholar
  8. Sherman F, Stewart JW, Parker J, Inhaber E, Shipman MA, Putterman GJ, Gardisky RL, Margoliash E (1968) J Biol Chem 243:5446–5456Google Scholar
  9. Slonimski PP, Acher R, Péré G, Sets A, Somlo M (1963) International Symposium on Mechanisms of Regulation of Cellular Activities in Microorganisms, C.N.R.S., Marseille. Gordon & Breach, New York, pp 435–461Google Scholar
  10. Verdière J, Petrochilo E (1975) Biochem Biophys Res Commun 67:1451–1458Google Scholar
  11. Verdière J, Petrochilo E (1979) Mol Gen Genet 175:209–216Google Scholar
  12. Verdière J, Creusot F, Guérineau M (1985) Mol Gen Genet 199:524–533Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • J. Verdière
    • 1
  • F. Creusot
    • 1
  • L. Guarente
    • 2
  • P. P. Slonimski
    • 1
  1. 1.Centre de Génétique Moléculaire du C. N. R. S.Laboratoire pro pre associé a l'Université Pierre et Marie CurieGif-sur-YvetteFrance
  2. 2.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

Personalised recommendations