Advertisement

Human Genetics

, Volume 68, Issue 3, pp 228–234 | Cite as

Fanconi anaemia cells are not uniformly deficient in unhooking of DNA interstrand crosslinks, induced by mitomycin C or 8-methoxypsoralen plus UVA

  • E. H. A. Poll
  • F. Arwert
  • H. T. Kortbeek
  • A. W. Eriksson
Original Investigations

Summary

Fanconi anaemia (FA) cells are extremely sensitive to crosslinking agents, e. g. mitomycin C, but only moderately sensitive to trimethylpsoralen plus UVA. Evidence has been reported suggesting that there is a deficient DNA crosslink repair mechanism in FA cells, but others failed to confirm this conclusion using other methods and other crosslinking agents. We reinvestigated the mitomycin C and 8-methoxypsoralen crosslink repair in FA cells with a high sensitivity to mitomycin C. Although an essentially similar methodology was used to that previously described, no difference between the control and FA cell strains was observed, neither for mitomycin C- nor for 8-methoxypsoralen-induced crosslinks.

Keywords

Internal Medicine Metabolic Disease Mitomycin Crosslinking Agent Repair Mechanism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Auerbach, AD, Wolman SR (1976) Susceptibility of Fanconi's anaemia fibroblasts to chromosome damage by carcinogens. Nature 261:494–496Google Scholar
  2. Bredberg A, Lambert B, Söderhäll S (1982) Induction and repair of psoralen crosslinks in DNA of normal human and xeroderma pigmentosum fibroblasts. Mutat Res 931:221–234Google Scholar
  3. Fanconi G (1967) Familial constitutional panmyelopathy: Fanconi's anemia (FA). I. Clinical aspects Semin Hematol 4:233–240Google Scholar
  4. Fornace AJ Jr, Little JB, Weichselbaum RR (1979) DNA repair in a Fanconi's anemia fibroblast cell strain. Biochim Biophys Acta 561:99–109Google Scholar
  5. Fujiwara Y, Tatsumi M, Sasaki MS (1977) Crosslink repair in human cells and its possible defect in Fanconi's anemia cells. J Mol Biol 113:635–649Google Scholar
  6. Fujiwara Y (1982) Defective repair of mitomycin c crosslinks in Fanconi's anemia and loss in confluent normal human and xeroderma pigmentosum cells. Biochim Biophys Acta 699:217–225Google Scholar
  7. Glanz A, Fraser F (1982) Spectrum of anomalies in Fanconi anemia. J Med Genet 19:412–416Google Scholar
  8. Iyer VN, Szybalski W (1964) Mitomycins and porfyromycin: chemical mechanism of activation and crosslinking of DNA. Science 145:55–58Google Scholar
  9. Kano Y, Fujiwara Y (1982) Dyskeratosis congenita: survival, sister chromatid exchange and repair following treatments with crosslinking agents Mutat Res 103:327–332Google Scholar
  10. Kaye J, Smith C, Hanawalt PC (1980) DNA repair in human cells containing photoadducts of 8-methoxypsoralen or angelicin. Cancer Res 40:696–702Google Scholar
  11. Kwee ML, Poll EHA, van de Kamp JJP, de Koning H, Eriksson AW, Joenje H (1983) Unusual response to bifunctional alkylating agents in a case of Fanconi anaemia. Hum Genet 64:384–387Google Scholar
  12. Lipsett MN, Weissbach A (1965) The site of alkylation of nucleic acids by mitomycin c. Biochemistry 4:206–211Google Scholar
  13. Poll EHA, Arwert F, Joenje H, Eriksson AW (1982) Cytogenetic toxicity of antitumor platinum compounds in Fanconi's anemia. Hum Genet 61:228–230Google Scholar
  14. Sasaki MS, Tonomura M (1973) A high susceptibility of Fanconi's anemia to chromosome breakage by DNA crosslinking agents. Cancer Res 33:1829–1836Google Scholar
  15. Sasaki MS (1975) Is Fanconi's anemia defective in a process essential to the repair of DNA crosslinks? Nature 257:501–503Google Scholar
  16. Sasaki MS (1978) Fanconi's anemia condition possibly associated with a defective DNA repair. In: Hanawalt PC, Friedberg EC, Fox CF (eds) DNA repair mechanisms. ICN-UCLA Symposia on molecular and cellular biology, vol IX, p 675Google Scholar
  17. Schroeder TM, Anschütz F, Knopp A (1964) Spontane Chromosomenaberrationen bei familiärer Panmyelopathie. Humangenetik 1:194–196Google Scholar
  18. Schroeder TM, Kurth R (1971) Spontaneous chromosomal breakage and high incidence of leukemia in inherited disease. Blood 37:96–112Google Scholar
  19. Schroeder TM (1982) Genetically determined chromosome instability syndromes. Cytogenet Cell Genet 33:119–132Google Scholar
  20. Song PS, Tapley KJ Jr (1979) Photochemistry and photobiology of psoralens. Photochemistry and Photobiology 29:1177–1197Google Scholar
  21. Sognier MA, Hittelman WN (1983) Loss of repairability of DNA interstrand crosslinks in Fanconi's anemia cells with culture age. Mutat Res 108:383–393Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • E. H. A. Poll
    • 1
  • F. Arwert
    • 1
  • H. T. Kortbeek
    • 1
  • A. W. Eriksson
    • 1
  1. 1.Institute of Human Genetics, Faculty of MedicineFree UniversityAmsterdamThe Netherlands

Personalised recommendations