Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells

  • 44 Accesses

  • 7 Citations

Summary

Co-culturing with origin-defective SV40 DNA-injected transformants, we succeeded in activating the limited growth potential of late-phase-II fibroblasts from patients with Werner's syndrome (WS cells). Residual life spans of three WS cell lines increased 2.3–5.6 fold. Co-culture with normal IMR-90 cells showed variable effects on different WS cell lines.

This is a preview of subscription content, log in to check access.

References

  1. Gluzman Y, Frisque RJ, Sambrook J (1979) Origin-defective mutants of SV-40. Cold Spring Harbor Symp on Quant Biol 44:293–300

  2. Noda K, Umeda M, Ono T (1984) Transforming growth factors in human colostrum. Gann 75:109–112

  3. Salk D (1982) Werner's syndrome: A review of recent research with an analysis of connective tissue metabolism, growth control of cultured cells, and chromosomal aberrations. Hum Genet 62:1–15

  4. Small MB, Gluzman Y, Ozer HL (1982) Enhanced transformation of human fibroblasts by origin-defective simian virus 40. Nature 296:671–672

  5. Yamamoto F, Furusawa M, Furusawa I, Obinata M (1982) The pricking method: A new efficient technique for mechanically introducing foreign DNA into the nuclei of culture cells. Exp Cell Res 142:79–84

  6. Yamane I, Kan M, Hoshi H, Minamoto Y (1981) Primary culture of human diploid cells and its long-term transfer in serum-free culture medium. Exp Cell Res 134:470–474

Download references

Author information

Correspondence to T. Ohno.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ohno, T., Yamaguchi, N. Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Hum Genet 68, 209–210 (1984). https://doi.org/10.1007/BF00418390

Download citation

Keywords

  • Internal Medicine
  • Metabolic Disease
  • Life Span
  • Variable Effect
  • Limited Growth