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Current Genetics

, Volume 10, Issue 8, pp 573–578 | Cite as

Cloning of a Candida utilis gene which complements leu2 mutation in Saccharomyces cerevisiae

  • Y. Zheng Zhang
  • C. Adinarayana Reddy
Original Articles

Summary

DNA fragments containing the LEU2 gene of Candida utilis have been isolated, utilizing the genome library (constructed in YRp12) of this organism. Two recombinant plasmids pZR84 and pZR32, containing the cloned LEU2 gene, were 4.24 kb and 10.4 kb, respectively, and were shown to complement leu2 mutation in Saccharomyces cerevisiae and leuB mutation in Escherichia coli. The cloned fragment in pZR84 contained one restriction site each for EcoRI and PvuII, and two for HindIII, but none for SalI, BamHI or Pstl. This cloned fragment hybridized with the total DNA from C. utilis and from Leu+ transformants of S. cerevisiae, but not with that from untransformed S. cerevisiae. Subcloning analyses showed that a 2.34 kb BamHI HindIII fragment of the cloned C. utilis sequence contains the region essential for the expression of the LEU2 gene.

Key words

Candida utilis Cloning LEU2 gene YRp 12 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Y. Zheng Zhang
    • 1
  • C. Adinarayana Reddy
    • 1
  1. 1.Department of Microbiology and Public HealthMichigan State UniversityEast LansingUSA

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