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Effects of cordycepin, hydroxyurea and cycloheximide on histone mRNA synthesis in synchronized HeLa cells

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Abstract

The effects of cordycepin, hydroxyurea and cycloheximide on the synthesis of histone mRNA in synchronized HeLa cells were studied by quantitating the RNA, using translation in a rabbit reticulocyte cell-free system. It was found that biologically active histone mRNA is synthesized in the presence of cordycepin. This result strengthens the findings by others that histone mRNA does not contain poly(A). Hydroxyurea and cycloheximide, when used at concentrations that inhibit cellular DNA synthesis, had different effects on histone messenger activity. While polyribosomal messenger activity rapidly declined after addition of hydroxyurea it was not impaired by cycloheximide. These results might help to elucidate regulatory mechanisms involved in the coupled synthesis of DNA and histones in HeLa cells.

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Breindl, M., Gallwitz, D. Effects of cordycepin, hydroxyurea and cycloheximide on histone mRNA synthesis in synchronized HeLa cells. Mol Biol Rep 1, 263–268 (1974). https://doi.org/10.1007/BF00417581

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Keywords

  • HeLa Cell
  • Regulatory Mechanism
  • Cycloheximide
  • Hydroxyurea
  • mRNA Synthesis