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A novel X-linked member of the human zinc finger protein gene family: isolation, mapping, and expression

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We report the partial characterization of a novel putative zinc finger gene of the Krüppel-type (ZNF81), isolated from an X Chromosome (Chr) specific library. The pattern of segregation in human-hamster somatic cell hybrids of sequences homologous to the ZNF81 finger domain has established that it resides within the Xp22.1-Xp11 region. ZNF81 represents yet another example, together with ZFX, ZNF41, and ZNF21, of members of the zinc finger gene family residing within the short arm of the human X Chr. Sequence analysis showed that ZNF81 may encode a polypeptide(s) containing tandem arrays of 12 canonical C2H2 zinc fingers of the Krüppel-type at the C-terminus. Northern analysis indicated that probes from the ZNF81 finger domain hybridize to polyadenylated transcripts present in several cell lines, a result that supports the hypothesis that it is an expressed, functional member of this multigene family.

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Correspondence to Giovanna Grimaldi.

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Marino, M., Archidiacono, N., Franzé, A. et al. A novel X-linked member of the human zinc finger protein gene family: isolation, mapping, and expression. Mammalian Genome 4, 252–257 (1993).

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  • Zinc Finger
  • Multigene Family
  • Cell Hybrid
  • Finger Protein
  • Zinc Finger Protein