The rate of synthesis and degradation of phospholipids in Mycobacterium smegmatis ATCC 607, grown at 27° C and 37° C was studied by incorporation of 32P into phospholipids and chase of radioactivity of the pulse-labelled phospholipids. A relatively low rate of synthesis and degradation of phospholipids in cells growth at 27° C was observed as compared to those grown at 37° C. Phosphatidylethanolamine (PE) had the maximum turnover at 37° C. However, at 27° C, cardiolipin (CL) showed a turnover rate higher than PE. Phosphatidylinositol mannosides (PIMs) were metabolically more active at 37° C than at 27° C. The differences in metabolic activity of the phospholipids at the two temperatures have been discussed.
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- M. 607:
Mycobacterium smegmatis ATCC 607
Akamatsu Y, Ono Y, Nojima S (1966) Phospholipid patterns in subcellular fractions of Mycobacterium phlei. J Biochem (Tokyo) 59:176–182
Akamatsu Y, Ono Y, Nojima S (1967) Studies on the metabolism of phospholipids in Mycobacterium phlei. I. Differences in the turnover rates of individual phospholipids. J Biochem (Tokyo) 61:96–102
Bright-Gaertner E, Proulx P (1972) Metabolism of phosphoglycerides in Escherichia coli during growth at 37° C and a cold-induced lag phase. Biochim Biophys Acta 270:40–49
Cronan JE, Vagelos PR (1972) Metabolism and function of the membrane phospholipids of Escherichia coli. Biochim Biophys Acta 265:25–60
Cronan JE, Jr (1978) Molecular biology of bacterial membrane lipids. Ann Rev Biochem 47:163–189
Dhariwal KR, Chander A, Venkitasubramanian TA (1978) Turnover of lipids in Mycobacterium smegmatis CDC 46 and Mycobacterium phlei ATCC 354. Arch Microbiol 116:69–75
Farrell J, Rose AH (1967) Temperature effects on microorganisms. Ann Rev Microbiol 21:101–115
Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509
Goldfine H (1972) Comparative aspects of bacterial lipids. Adv Microbiol Physiol 8:1–58
Goren MB (1972) Mycobacterial lipids: Selected topics. Bacteriol Rev 36:32–63
Marinetti GV (1962) Chromatographic separation, identification and analysis of phosphatides. J Lipid Res 3:1–20
McElhaney RN (1976) Mechanisms of microbial adaptation. In: Heinrich MR (ed) Extreme environments. Academic Press, New York, pp 255–281
Minnikin DE, Abdolrahimzadeh H, Baddiley J (1974) The occurrence of phosphatidyl-ethanolamine and glycosyl-diglycerides in thermophilic bacilli. J Gen Microbiol 83:415–418
Okuyama H (1969) Phospholipid metabolism in Escherichia coli after a shift in temperature. Biochim Biophys Acta 176:125–134
Ramakrishnan T, Murthy PS, Gopinathan KP (1972) Intermediary metabolism of mycobacteria. Bact Rev 36:65–108
Reddy TLP, Venkitasubramanian TA (1975) Metabolism of mycobacteria. J Sci Indus Res 34:319–328
Rothfield L, Finkelstein A (1968) Membrane biochemistry. Ann Rev Biochem 37:463–496
Subrahmanyam D (1965) Studies on the metabolism of phosphatides of Mycobacterium 607. Ind. J. Biochem 2:27–30
Talwar P, Khuller GK (1977) Lipids of Streptomyces griseus. Indian J Biochem Biophys 14:72–74
Taneja R, Malik U, Khuller GK (1979) Effect of growth temperature on the lipid composition of Mycobacterium smegmatis ATCC 607. J Gen Microbiol 113:413–416
Taneja R, Khuller GK (1980) Ethanol-induced alterations in phospholipids and fatty acids of Mycobacterium smegmatis ATCC 607. FEMS Lett. 8:83–85
Trana AK, Khuller GK, Subrahmanyam D (1980) Metabolism of phospholipids in Nocardia polychromogenes: J Gen Microbiol 116:89–92
Verma JN, Khera A, Khuller GK, Subrahmanyam D: (1980) Phosphoglycerides metabolism in Streptomyces griseus. Current Microbiol 4:13–16
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Taneja, R., Khuller, G.K. Phospholipid metabolism in Mycobacterium smegmatis ATCC 607 grown at 37° C and 27° C. Arch. Microbiol. 129, 81–84 (1981). https://doi.org/10.1007/BF00417185
- Phosphatidylinositol mannosides
- Mycobacterium smegmatis ATCC 607