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Phospholipid metabolism in Mycobacterium smegmatis ATCC 607 grown at 37° C and 27° C

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Abstract

The rate of synthesis and degradation of phospholipids in Mycobacterium smegmatis ATCC 607, grown at 27° C and 37° C was studied by incorporation of 32P into phospholipids and chase of radioactivity of the pulse-labelled phospholipids. A relatively low rate of synthesis and degradation of phospholipids in cells growth at 27° C was observed as compared to those grown at 37° C. Phosphatidylethanolamine (PE) had the maximum turnover at 37° C. However, at 27° C, cardiolipin (CL) showed a turnover rate higher than PE. Phosphatidylinositol mannosides (PIMs) were metabolically more active at 37° C than at 27° C. The differences in metabolic activity of the phospholipids at the two temperatures have been discussed.

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Abbreviations

M. 607:

Mycobacterium smegmatis ATCC 607

32P:

32P-orthophosphate

PIMs:

phosphatidylinositol mannosides

PE:

phosphatidylethanolamine

CL:

cardiolipin

TCA:

trichloroacetic acid

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Taneja, R., Khuller, G.K. Phospholipid metabolism in Mycobacterium smegmatis ATCC 607 grown at 37° C and 27° C. Arch. Microbiol. 129, 81–84 (1981). https://doi.org/10.1007/BF00417185

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Key words

  • Incorporation
  • Synthesis
  • Chase
  • Turnover
  • Phosphatidylinositol mannosides
  • Phosphatidylethanolamine
  • Cardiolipin
  • Mycobacterium smegmatis ATCC 607
  • 32P-orthophosphate