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Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium

Abstract

Anaerobic sea or fresh water media with acetate and elemental sulfur yielded enrichments of a new type of strictly anaerobic, rod-shaped, laterally flagellated, Gram-negative bacterium. Three pure culture-strains from different sulfide-containing sea water sources were characterized in detail and are described as a new genus and species Desulfuromonas acetoxidans.

The new bacterium is unable to ferment organic substances; it obtains energy for growth by anaerobic sulfur respiration. Acetate, ethanol or propanol can serve as carbon and energy source for growth; their oxidation to CO2 is stoichiometrically linked to the reduction of elemental sulfur to sulfide. Organic disulfide compounds, malate or fumarate are the only other electron acceptors used. Butanol and pyruvate are used in the presence of malate only; no other organic compounds are utilized. Biotin is required as a growth factor. The following dry weight yields per mole of substrate are obtained: in the presence of sulfur: 4.21 g on acetate, 9.77 g on ethanol; in the presence of malate: 16.5 g on acetate, 34.2 g on ethanol and 46.2 g on pyruvate. Accumulations of cells are pink; cell suspensions exhibit absorption spectra resembling those of c-type cytochromes (abs. max. at 419, 523 and 553 nm). Malate-ethanol grown cells contain a b-type cytochrome in addition.

In the presence of acetate, ethanol or propanol, Desulfuromonas strains form robust growing syntrophic mixed cultures with phototrophic green sulfur bacteria.

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References

  1. Abd-el Malek, Y., Rizk, S. G.: Counting of sulfate-reducing bacteria in mixed bacterial populations. Nature (Lond.) 182, 538 (1958)

  2. Barker, H. A.: ATP formation by anaerobic bacteria. In: Horizons of bioenergetics (A. San Pietro, H. Gest, eds.), pp. 7–31. New York: Academic Press 1972

  3. Conn, H. J.: Use of the microscope in studying the activities of bacteria in soil. J. Bact. 17, 399–405 (1929)

  4. van Gemerden, H.: On the ATP-generation by Chromatium in darkness, Arch. Mikrobiol. 64, 118–124 (1968)

  5. Gottschalk, G.: The sterospecifity of the citrate synthase in sulfate-reducing and photosynthetic bacteria. Europ. J. Biochem. 5, 346–351 (1968)

  6. Gray, B. H., Fowler, C. F., Nugent, N. A., Rigopoulos, N., Fuller, R. C.: Reevaluation of Chloropseudomonas ethylica strain 2-K. Int. J. Syst. Bact. 23, 256–264 (1973)

  7. Hansen, T. A., van Gemerden, H.: Sulfide utilization by purple nonsulfur bacteria. Arch. Mikrobiol. 86, 49–56 (1972)

  8. Harder, W., van Dijken, J. P.: Theoretical considerations on the relation between energy production and growth of methaneutilizing bacteria. Symp. on microbial production and utilization of atmospheric trace gases 1975. Göttingen: Goltze 1976

  9. Hatchikian, E. C., Le Gall, J.: Evidence for the presence of a b-type cytochrome in the sulfate-reducing bacterium Desulfovibrio gigas, and its role in the reduction of fumarate by molecular hydrogen. Biochim. biophys. Acta (Amst.) 267, 479–484 (1972)

  10. Holz, G., Bergmeyer, H. U.: Acetatbestimmung mit Acetatkinase und Hydroxylamin. In: Methoden der enzymatischen Analyse (H. U. Bergmeyer, ed.). Weinheim: Verlag Chemie 1974

  11. Jacobs, N. J., Wolin, M. J.: Electron transport system of Vibrio succinogenes. I. Enzymes and cytochromes of the electrontransport system. Biochim. biophys. Acta (Amst.) 69, 18–28 (1963)

  12. Jannasch, H. W.: Zur Methodik der quantitativen Untersuchung von Bakterienkulturen in flüssigen Medien. Arch. Mikrobiol. 18, 425–430 (1953)

  13. Klemme, J.-H., Schlegel, H. G.: Untersuchungen zum Cytochrom-Oxydase-System aus anaerob im Licht und aerob im Dunkeln gewachsenen Zellen von Rhodopseudomonas capsulata. Arch. Mikrobiol. 68, 326–354 (1969)

  14. Larsen, H.: On the microbiology and biochemistry of photosynthetic green sulfur bacteria. Kgl. Norske Videnskab. Selskabs Skrifter, No. 1, 199 pp. (1953)

  15. Lewis, A. J., Miller, J. D. A.: Keto acid metabolism in Desulfovibrio. J. gen. Microbiol. 90, 286–292 (1975)

  16. van Niel, C. B.: On the morphology and physiology of the purple and green sulfur bacteria. Arch. Mikrobiol. 3, 1–112 (1932)

  17. Pfennig, N.: Anreicherungskulturen für rote und grüne Schwefelbakterien. In: Anreicherungskultur und Mutantenauslese (H. G. Schlegel, ed.). Zbl. Bakt., I. Abt. Orig., Suppl. 1, 179–189, 503–504 (1965)

  18. Pfennig, N., Lippert, K.-D.: Über das Vitamin B12-Bedürfnis phototropher Schwefelbakterien. Arch. Mikrobiol. 55, 245–256 (1966)

  19. Postgate, J. R.: The reduction of sulphur compounds by Desulphovibrio desulphuricans. J. gen. Microbiol. 5, 725–738 (1951)

  20. Postgate, J. R.: Cytochrome c 3 and desulphoviridin; pigments of the anaerobe Desulphovibrio desulphuricans. J. gen. Microbiol. 14, 545–572 (1956)

  21. Roy, A. B., Trudinger, P. A.: The biochemistry of inorganic compounds of sulphur. Cambridge: University Press 1970

  22. Siefert, E.: Die Fixierung von molekularem Stickstoff bei phototrophen Bakterien am Beispiel von Rhodopseudomonas acidophila. Dissertation, Universität Göttingen (1976)

  23. Trüper, H. G., Pfennig, N.: Sulphur metabolism in Thiorhodaceae. III. Storage and turnover of thiosulfate sulphur in Thiocapsa floridana and Chromatium species. Antonie v. Leeuwenhoek 32, 261–276 (1966)

  24. Trüper, H. G., Schlegel, H. G.: Sulphur metabolism in Thiorhodaceae. I. Quantitative measurements of growing cells of Chromatium okenii. Antonie v. Leeuwenhoek 30, 225–238 (1964)

  25. de Vries, W., van Wijk-Kapteyn, W. M. C., Oosterhuis, S. K. H.: The presence and function of cytochromes in Selenomonas ruminantium, Anaerovibrio lipolytica and Veillonella alcalescens. J. gen. Microbiol. 81, 69–78 (1974)

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Dedicated to Prof. Roger Y. Stanier on the occasion of his 60th barthday

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Pfennig, N., Biebl, H. Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium. Arch. Microbiol. 110, 3–12 (1976). https://doi.org/10.1007/BF00416962

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Key words

  • Desulfuromonas acetoxidans
  • Species description
  • Anaerobic acetate oxidation
  • Sulfur reduction
  • Ethanol
  • Propanol
  • Hydrogen sulfide
  • Cytochromes
  • Growth yield