Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The growth and partition of cell membranes during synchronized division cycle of Caulobacter crescentus

  • 58 Accesses

Summary

The growth and division of cell membranes in Caulobacter crescentus has been studied. This microorganism divides into flagellated and stalked cells which are easily separated by centrifugation. The biosynthesis and partition of membranes was studied by labeling the proteins with [3H]-leucine and the lipids with [32P]. The membranes were prepared from cell spheroplasts. They further purified on a sucrose gradient.

The data obtained show changes of the rate of synthesis of membranes in C. crescentus during the first synchronized division cycle (110 min): the rate is faster in the first 70 min and it drops by 26% during the following 40 min. During the period of faster synthesis the flagellated cells are changing into stalked cells while doubling in size.

There is also an intracellular pool of membrane precursors the quantity of which almost doubles as the rate of membrane synthesis decreases, i.e., before cell division.

The macromolecules constituting the membranes are not degraded.

After division, in each membrane of the two morphologically different cell types the specific radioactivity is 50% of that of the parent cell membranes.

This is a preview of subscription content, log in to check access.

References

  1. Folch, J., Lees, M., Stanley, G.: A simple method for isolation and purification of total lipids from animal tissue. J. biol. Chem. 226, 497–509 (1957)

  2. Kahane, I., Razin, S.: Synthesis and turnover of membrane protein and lipid in Mycoplasma laidlawii. Biochim. biophys. Acta. (Amst.) 183, 79–89 (1969)

  3. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

  4. Mandelstam, J.: Turnover of protein in growing and non-growing populations of Escherichia coli. Biochem. J. 69, 110–119 (1958)

  5. Matile, P. H., Moor, H., Mühlethaler, K.: Isolation and properties of the plasmalemma in yeast. Arch. Mikrobiol. 58, 201–211 (1967)

  6. Nishi, A., Kogoma, T.: Protein turnover in the cell cycle of Escherichia coli. J. Bact. 90, 884–890 (1965)

  7. Schnaitman, C. A.: Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J. Bact. 104, 890–901 (1970)

  8. Shapiro, L., Agabian-Keshishian, N.: Specific assay for differentiation in the stalked bacterium Caulobacter crescentus. Proc. nat. Acad. Sci. (Wash.) 67, 200–203 (1970)

  9. Stove, J. L., Stanier, R. Y.: Cellular differentiation in stalked bacteria. Nature (Lond.) 196, 1189–1192 (1962)

  10. Warren, L., Glick, M. C.: Membranes of animal cells. II. The metabolism and turnover of membranes. J. Cell Biol. 37, 729–746 (1968)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Galdiero, F. The growth and partition of cell membranes during synchronized division cycle of Caulobacter crescentus . Archiv. Mikrobiol. 94, 125–132 (1973). https://doi.org/10.1007/BF00416687

Download citation

Keywords

  • Lipid
  • Sucrose
  • Cell Membrane
  • Centrifugation
  • Cell Division