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Thermostable extracellular protease of Bacillus stearothermophilus: factors affecting its production

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A strain of protease-producing Bacillus stearothermophilus has been isolated. Glycerol was the best carbon source for production whereas yeast extract was the best nitrogen source. The bacterium could grow up to 70°C but optimum protease production was at 60°C. Best initial pH for protease production was 5. Alkaline pH inhibited production. The enzyme was stable at 60°C for 18 h and was inhibited by EDTA, PMSF and HgCl2.

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The authors are with the Enzyme and Microbial Technology Group, Faculty of Science and Environmental Studies, Universiti Pertanian Malaysia, 43400 UPM Serdang, Selangor, Malaysia

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Razak, N.A., Samad, M.Y.A., Basri, M. et al. Thermostable extracellular protease of Bacillus stearothermophilus: factors affecting its production. World Journal of Microbiology & Biotechnology 10, 260–263 (1994).

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Key words

  • Bacillus stearothermophilus
  • protease