Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Flow of 14C-methanol via assimilatory and dissimilatory sequences with yeast in presence of glucose

  • 27 Accesses

  • 10 Citations

Abstract

Experiments were performed to reveal the extent to which individual heterotrophic substrates of a mixture contribute to the overall carbon and energy metabolism. For this reason Hansenula polymorpha MH 20 was chemostatically (C-limited) cultivated at different growth rates on mixtures of methanol and glucose fed at proportions of 3:1 and 1:3 (in weight units), respectively. The distributions of 14C-carbon from methanol in biomass as well as carbon dioxide (and supernatant) fractions were determined. From these results it followed, firstly, that energy derived from methanol dissimilation was used in part for the incorporation of glucose carbon, resulting in carbon conversion efficiencies for this substrate equivalent to yield coefficients of 0.61–0.69 g/g. Secondly, the growth yield data revealed that the efficiency of methanol conversion had to be increased in order to account for the experimentally determined yield figures. This was further confirmed by theoretical treatment of the growth yield data which showed that these could only be obtained if P/O-quotients for methanol conversion similar to those for glucose, i.e. 2.0–2.5, were considered. The latter property was regarded as the main reason for the observed improvement of growth yield accompanying the simultaneous utilization of methanol and glucose in this yeast.

This is a preview of subscription content, log in to check access.

Abbreviations

ATPM,a :

ATP required for incorporation of assimilated methanol at a given P/O-quotient

ATPM,d :

ATP generated from dissimilated methanol at a given P/O-quotient

G and M:

glucose and methanol; respectively (the indices u, a, d and e mean utilized, assimilated, dissimilated and incorporated by excess energy, respectively)

PGA:

3-phosphoglyceric acid

Y G app :

apparent growth yield on glucose in presence of methanol

Y G P/O :

theoretical growth yield on glucose at a given P/O-quotient

References

  1. Anthony C (1978) The prediction of growth yields in methylotrophs. J Gen Microbiol 104:91–104

  2. Babel W (1979) Bewertung von Substraten für das mikrobielle Wachstum auf der Grundlage ihres Kohlenstoff/Energie-Verhältnisses. Z Allg Mikrobiol 19:671–677

  3. Babel W (1983) The auxiliary substrate concept — an approach to improving the growth yield in SCP production. in: Halama DV (ed) Proceedings of the 3rd Symp Soc Countries on Biotechnology, Bratislava, CSSR, April 25–29, pp 169–176

  4. Babel W, Müller RH (1985a) Mixed substrate utilization in microorganisms: biochemical aspects and energetics. J Gen Microbiol 131:39–46

  5. Babel W, Müller RH (1985b) Correlation between cell composition and carbon conversion efficiency. A theoretical study. Appl Microbiol Biotechnol 222:201–207

  6. Babel W, Müller RH (1985c) The influence of carbon catabolism on the auxiliary substrate effect. Acta Biotechnol (in press)

  7. Eggeling L, Sahm H (1981) Enhanced utilization-rate of methanol during growth on a mixed substrate: a continuous culture study with Hansenula polymorpha. Arch Microbiol 130:362–365

  8. Egli T (1980) Wachstum von Methanol assimilierenden Hefen. Eidgenössische Technische Hochschulze Zürich. Thesis No 6538

  9. Egli T, Käppeli O, Fiechter A (1982) Regulatory flexibility of methylotrophic yeasts in chemostat culture: simultaneous assimilation of glucose and methanol at a fixed dilution rate. Arch Microbiol 131:1–7

  10. Egli T, Lindley ND, Quayle JR (1938) Regulation of enzyme synthesis and variation of residual methanol concentration during carbon-limited growth of Kloeckera sp. 2201 on mixtures of methanol and glucose. J Gen Microbiol 129:1269–1281

  11. Held Schlanderer G, Reimann J, Deilweg H (1978) Continuous culture of Candida boidinii variant 60 growing on methanol. Eur J Appl Microbiol Biotechnol 6:127–132

  12. Müller RH, Babel W (1984) Glucose as an auxiliary substrate. The influence of its carbon catabolism on the maximum carbon conversion efficiency. Appl Microbiol Biotechnol 20:195–200

  13. Müller RH, Markuske KD, Babel W (1983) Verbesserung der Y-Werte von Hansenula polymorpha auf Methanol durch simultane Verwertung von Glucose. Z Allg Mikrobiol 23:375–384

  14. Pilat P, Prokop A (1975) The effect of methanol, formaldehyde, and formic acid on growth of Candida boidinii 11 Bh. Biotechnol Bioeng XVII:1717–1723

  15. Stouthamer AH (1973) A theoretical study on the amount of ATP required for the synthesis of microbial cell material. Antonie van Leeuwenhoek J Microbiol Serol 39:545–565

  16. Von Jagow G, Klingenberg M (1970) Pathways of hydrogen in mitochondria of Saccharomyces carlsbergensis. Eur J Biochem 12:583–592

  17. Van Verseveld HW, Boon JP, Stouthamer AH (1979) Growth yields and the efficiency of oxidative phosphorylation of Paracoccus denitrificans during two-(carbon) substrate-limited growth. Arch Microbiol 121:213–223

Download references

Author information

Correspondence to Roland H. Müller.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Müller, R.H., Uhlenhut, G.J. & Babel, W. Flow of 14C-methanol via assimilatory and dissimilatory sequences with yeast in presence of glucose. Arch. Microbiol. 143, 77–81 (1985). https://doi.org/10.1007/BF00414772

Download citation

Key words

  • Mixed substrate utilization
  • 14C-Methanol glucose
  • Efficiency of methanol dissimilation
  • Improvement of growth yield
  • Hansenula polymorpha