The lipid composition of S. cerevisiae cells was studied in different conditions of pH and of HCO3 - and CO2 concentration.
Increasing the concentration of CO2 in the broth determines an increase of total lipids, of total fatty acids, and of the relative amount of unsaturated fatty acids.
Changing the concentration of bicarbonates at constant pCO2 does not affect the lipid composition of the cells. Also pH changes do not have significant effects.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Burchfield, H. P., and E. E. Storrs: Biochemical applications of gas chromatography. New York-London: Academic Press 1962.
Calvin, M., and N. G. Pon: Carboxylations and decarboxylations. J. cell. comp. Physiol. 54 (Suppl. 1), 51–74 (1959).
Folch, J., M. Less, and G. H. S. Stanley: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1957).
Kaziro, Y., L. F. Hass, P.D. Boyer, and S. Ochoa: Mechanism of the propionil-carboxylase Reaction. J. biol. Chem. 237, 1460–1468 (1962).
Klein, H. P.: Some observations on a free lipid synthesizing system from Saccharomyces cerevisiae. J. Bact. 73, 530–537 (1957).
—: Yeast particle fraction involved in lipid synthesis. J. Bact. 80, 665–672 (1960).
Lynen, F.: Biosynthesis of saturated fatty acids. Fed. Proc. 20, 941–951 (1961).
—: The role of Biotin-dependent carboxylations in biosynthetic reactions. Biochem. J. 102, 381–400 (1967).
Longmore, W. J., A. B. Hastings, and E. S. Harrison: The effect of physiological variations in pH and CO2 concentrations on acetate-1-C14 metabolism. Proc. nat. Acad. Sci. (Wash.) 52, 1040–1043 (1964).
Page, E., and L. Michaud: The titrimetric determination of plasma fatty acids. Canad. J. med. Sci. 29, 239–244 (1951).
Stoffel, W., F. Chu, and C. H. Arens: Analysis of long-chain fatty acids by gas-liquid chromatography. Analyt. Chem. 31, 307–308 (1959).
Utter, M. F.: The enzymes, Vol. 5. New York-London: Academic Press 1961.
Wakil, S. I., E. B. Titchener and D. M. Gibson: Evidence for the participation of biotin in the enzymic synthesis of fatty acids. Biochim. biophys. Acta (Amst.) 29, 225–226 (1958).
About this article
Cite this article
Castelli, A., Littarru, G.P. & Barbaresi, G. Effect of pH and CO2 concentration changes on lipids and fatty acids of Saccharomyces cerevisiae . Archiv. Mikrobiol. 66, 34–39 (1969). https://doi.org/10.1007/BF00414661
- Saccharomyces Cerevisiae