Archives of Microbiology

, Volume 137, Issue 3, pp 209–214 | Cite as

An examination of factors affecting the instability of Saccharomyces cerevisiae glucan synthetase in cell free extracts

  • F. Leal
  • J. Ruiz-Herrera
  • J. R. Villanueva
  • G. Larriba
Original Papers

Abstract

Yeast β(1–3) glucan synthetase is stimulated and stabilized by EDTA. Sucrose protects the enzyme from selfinactivaton. Preincubation of cell free extracts at low sucrose concentrations indicates a slow transition of the enzyme towards dissociation. Transition kinetics at 30° C and 0° C in the presence and in the absence of sucrose are interpreted assuming that a subunit is thermolabile in the free state and that sucrose increases its stability. Magnesium is deletereous for glucan synthetase in cell-free extracts. Chaotropic agents inactivate glucan synthetase according to their capacity to solubilize and depolymerize biological compounds. Fluoride plays a special role in the activation of glucan synthetase. Its action appears to be dependent on the presence of GTP (or other nucleotides). The role of all these agents on the activity and stability of the enzyme is interpreted in a unified scheme.

Key words

Saccharomyces cerevisiae Glucan synthetase EDTA Magnesium Sucrose Fluoride 

Abbreviations

EDTA

ethylene diamine tetraacetate

Tris

tris-(hydroxymethyl) aminomethane

MMF

mixed membrane fraction

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • F. Leal
    • 1
  • J. Ruiz-Herrera
    • 1
  • J. R. Villanueva
    • 1
  • G. Larriba
    • 2
  1. 1.Departmento de Microbiología, Facultad de CienciasUniversidad de SalamancaSpain
  2. 2.Departamento de Microbiología, Facultad de CienciasUniversidad de ExtremaduraBadajozSpain

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