The specific activity of cathepsin B-like, cathepsin D-like, and leucine aminopeptidase enzymes was measured in dormant, aging, and germinating spores of wild-type and mutant Dictyostelium discoideum.
The activity of leucine aminopeptidase was relatively constant during spore aging and spore germination. The level of cathepsin D-like activity was highest in young dormant spores but decreased during germination or aging.
The level of cathepsin B-like activity remained constant in wild-type spores which were aged for 13 days. The dormant spores of spontaneous germination mutants initially contained low levels of cathepsin B-like activity which increased during aging. Thus, there was no correlation between the level of endogenous cathepsin B activity and the ability to be autoactivated or heat-activated. The level of cathepsin B-like activity does not have a role in the generation of energy for the swelling stage of germination. Finally, the combined level of endogenous and exogenous cathepsin B activity increased more than 20-fold during the emergence of myxamoebae suggesting that the enzyme(s) may play a role at this development stage of germination.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bradford M (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Chan AH, Cotter DA (1982) The role and expression of β-glucosidase during spore germination of mutant and wild-type Dictyostelium discoideum. Can J Microbiol 28:740–748
Dahlberg KR, Cotter DA (1978) Autoactivation of spore germination in mutant and wild-type strains of Dictyostelium discoideum. Microbios 23:153–166
Ennis HL (1981) Changes in free amino acid levels during cellular slime mold spore and microcyst germination. Arch Biochem Biophys 209:371–375
Firtel RA, Brackenbury RW (1972) Partial characterization of several protein and amino acid metabolizing enzymes in the cellular slime mold Dictyostelium discoideum. Dev Biol 27:307–321
Fong D, Rutherford CL (1978) Protease activity during cell differentiation of the cellular slime mold, Dictyostelium discoideum. J Bacteriol 134:521–527
Hemmes DE, Kojima-Buddenhagen ES, Hohl HR (1972) Structural and enzymatic analysis of the spore wall layers in Dictyostelium discoideum. J Ultrastr Res 41:406–417
Jackson DP, Chan AH, Cotter DA (1982) Utilization of trehalose during Dictyostelium discoideum spore germination. Dev. Biol 90:369–374
Jones, T, deRenobales M, Pon N (1979) Cellulases released during germination of Dictyostelium discoideum spores. J Bacteriol. 137:752–757
North MJ (1978) Inhibition of acid proteinase from Dictyostelium discoideum. Biochem Soc Trans 6:400–403
North MJ, Harwood JM (1979) Multiple acid proteinases in the cellular slime mold, Dictyostelium discoideum. Biochim Biophys Acta 566:222–233
O'Day DH (1976) Acid protease activity during germination of microcysts of the cellular slime mold, Polysphondylium pallidum. J Bacteriol 125:8–13
Rosness P (1968) Cellulolytic enzymes during morphogenesis in Dictyostelium discoideum. J Bacteriol 96:639–645
Sussman M, Sussman R (1969) Patterns of RNA synthesis and enzyme accumulation and disapperance during cellular slime mold differentiation. In: Crawford LV, Stoker MGP (eds) Microbial growth. Symp Soc Gen Microbiol 19: 403–435
Tisa LS, Cotter DA (1980) Expression of glycosidase activities during germination of Dictyostelium discoideum spores. J Bacteriol 141:436–442
Toama MA, Raper KB (1967) Microcysts of the cellular slime mold, Polysphondylium pallidum. I. Factors influencing microcyst formation. J Bacteriol 94:1143–1149
Weiner E, Ashworth JM (1970) The isolation and characterization of lysosomal particles from myxamoebae of the cellular slime mold, Dictyostelium discoideum. Biochem J 118:505–512
Wright BE, Anderson ML (1960) Protein and amino acid turnover during differentiation in the slime mold. II. Incorporation of 35S-methionine into the amino acid pool and into protein. Biochim Biophys Acta 43:67–78
About this article
Cite this article
Jackson, D.P., Cotter, D.A. Expression of proteolytic enzymes during Dictyostelium discoideum spore germination. Arch. Microbiol. 137, 205–208 (1984). https://doi.org/10.1007/BF00414544
- Dictyostelium discoideum
- Cathepsin B
- Cathepsin D
- Leucine aminopeptidase