Archives of Microbiology

, Volume 137, Issue 3, pp 188–193

Selective inhibition of transition from sexual agglutination to zygote formation by ethyl N-phenylcarbamate in Saccharomyces cerevisiae

  • Satoshi Hasegawa
  • Naohiko Yanagishima
Original Papers


Effects of ethyl N-phenylcarbamate (EPC) on the mating reaction of Saccharomyces cerevisiae were studied, with special attention on the effect on the α pheromone action. EPC inhibited zygote formation at a concentration which promoted induction of sexual agglutinability. EPC enhanced agglutinability induction by α pheromone, but inhibited α-pheromone-induced formation of large pearshaped cells in a mating type. The enhancement of agglutinability induction was accompanied with increased production of a agglutination substance and inhibition of α pheromone inactivation. EPC arrested the cell cycle of a cells probably in the step controlled by CDC19, CDC35, cAMP etc., just before the step controlled by CDC28, α pheromone etc.

Key words

Agglutination substance α Pheromone Cell cycle Ethyl N-phenylcarbamate Mating reaction Microtubules Saccharomyces cerevisiae 



Ethyl N-phenylcarbamate


0.01 M phosphate buffer solution, pH 5.5


spindle pole body


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Satoshi Hasegawa
    • 1
  • Naohiko Yanagishima
    • 1
  1. 1.Biological Institute, Faculty of ScienceNagoya UniversityNagoyaJapan

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