Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The influence of temperature on glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase and the regulation of these enzymes in a mesophilic and a thermophilic cyanobacterium


The activities and kinetics of the enzymes G6PDH (glucose-6-phosphate dehydrogenase) and 6PGDH (6-phosphogluconate dehydrogenase) from the mesophilic cyanobacterium Synechococcus 6307 and the thermophilic cyanobacterium Synechococcus 6716 are studied in relation to temperature. In Synechococcus 6307 the apparent K m's are for G6PDH: 80μM (substrate) and 20μM (NADP+); for 6PGDH: 90μM (substrate) and 25μM (NADP+). In Synechococcus 6716 the apparent K m's are for G6PDH: 550μM (substrate) and 30μM (NADP+); for 6PGDH: 40μM (substrate) and 10μM (NADP+). None of the K m's is influenced by the growth temperature and only the K m's of G6PDH for G6P are influenced by the assay temperature in both organisms. The idea that, in general, thermophilic enzymes possess a lower affinity for their substrates and co-enzymes than mesophilic enzymes is challenged.

Although ATP, ribulose-1,5-bisphosphate, NADPH and pH can all influence the activities of G6PDH and 6PGDH to a certain extent (without any difference between the mesophilic and the thermophilic strain), they cannot be responsible for the total deactivation of the enzyme activities observed in the light, thus blocking the pentose phosphate pathway.

This is a preview of subscription content, log in to check access.



glucose-6-phosphate, dehydrogenase


6-phosphogluconate dehydrogenase








N-Tris (hydroxymethyl)-methylglycine


  1. Adkins SW, Gosling PG, Ross JD (1980) Glucose-6-phosphate dyhydrogenase and 6-phosphogluconic acid dehydrogenase of wild oat seeds. Phytochemistry 19:2523–2525

  2. Amelunxen RE, Murdock AL (1978) Microbial life at high temperatures: mechanisms and molecular aspects. In: Kushner DJ (ed) Microbial life in extreme environments. Academic Press, London, pp 217–278

  3. Anderson LE, Ashton AR, Mohamed AH, Scheibe R (1982) Light/dark modulation of enzyme activity in photosynthesis. Bio Science 32:103–107

  4. Biggins J (1969) Respiration in blue-green algae. J Bacteriol 99: 570–575

  5. Bornefeld T, Simonis W (1974) Effects of light, pH, and inhibitors on the ATP level of the blue-green alga Anacystis nidulans. Planta 115:309–318

  6. Brock TD (1967) Life at high temperatures. Science 158:1012–1019

  7. Brock TD (1978) Thermophilic microorganisms and life at high temperatures. Springer, Berlin Heidelberg New York

  8. Brown AH, Webster GC (1953) The influence of light on the rate of respiration in the blue-green alga Anabaena. Am J Bot 40:753–758

  9. Buchanan BB (1980) Role of light in the regulation of chloroplast enzymes. Annu Rev Plant Physiol 31:341–374

  10. Cseke C, Balogh A, Farkas GL (1981a) Redox modulation of glucose-6-P dehydrogenase in Anacystis nidulans and its ‘uncoupling’ by phage infection. FEBS Lett 126:85–88

  11. Cseke C, Balogh A, Ruzicska P, Farkas GL (1981b) Light-mediated oxidoreductive modulation of glucose-6-phosphate dehydrogenase (G6PDH) in Anacystis nidulans. In: Akoyunoglou G (ed) Photosynthesis. IV. Regulation of carbon metabolism. Balaban International Science Services, Philadelphia, pp 395–404

  12. Falkner G, Horner F, Werdan K, Heldt HW (1976) pH changes in the cytoplasm of the blue-green alga Anacystis nidulans caused by light-dependent proton flux into the thylakoid space. Plant Physiol 58:717–718

  13. Fredrick JF (1973) Differences of the primer-independent phosphorylase isoenzyme in thermophilic and mesophilic algae. Plant Cell Physiol 14:443–448

  14. Gibson J (1981) Movement of acetate across the cytoplasmic membrane of the unicellular cyanobacteria Synechococcus and Aphanocapsa. Arch Microbiol 130:175–179

  15. Gosling PG, Ross JD (1979) Characterization of glucose-6-phosphate dehydrogenase and 6-phosphogluconic acid dehydrogenase from hazel cotyledons. Phytochemistry 18:1441–1445

  16. Grossman A, McGowan RE (1975) Regulation of glucose-6-phosphate dehydrogenase in blue-green algae. Plant Physiol 55:658–662

  17. Ihlenfeldt MJA, Gibson J (1975) CO2 fixation and its regulation in Anacystis nidulans (Synechococcus). Arch Microbiol 102:13–21

  18. Larsson CM, Olsson T (1979) Firefly assay of adenine nucleotides from algae: comparison of extraction methods. Plant Cell Physiol 20:245–155

  19. Lendzian K, Bassham JA (1975) Regulation of glucose-6-phosphate dehydrogenase in spinach chloroplasts by ribolose-1,5-diphosphate and NADPH/NADP+ ratios. Biochim Biophys Acta 396:260–275

  20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

  21. Matthijs HCP, Scholts MJC, Schreurs H (1981) Photosynthesis and respiration in the cyanobacterium Plectonema boryanum. In: Akoyunoglou G (ed) Photosynthesis. II. Electron transport and photophosphorylation. Balaban International Science Services, Philadelphia, pp 269–278

  22. Owens O, Krauss RW (1972) Kinetics of photophosphorylation in cells of Anacystis nidulans. Plant Physiol 49:52S

  23. Peary JA, Castenholz RW (1964) Temperature strains of a thermophilic blue-green algae. Nature (London) 202:720–721

  24. Pelroy RA, Bassham JA (1972) Photosynthetic and dark carbon metabolism in unicellular blue-green algae. Arch Microbiol 86:25–38

  25. Pelroy RA, Rippka R, Stanier RY (1972) Metabolism of glucose by unicellular blue-green algae. Arch Microbiol 87:303–322

  26. Pelroy RA, Kirk MR, Bassham JA (1976) Photosystem II regulation of macromolecule synthesis in the blue-green alga Aphanocapsa 6714. J. Bacteriol 128:623–632

  27. Preiss J, Levi C (1979) Metabolism of starch in leaves. In: Gibbs M, Latzko E (eds) Encyclopedia of plant physiology (new series), vol 6. Springer, Berlin Heidelberg New York, pp 282–312

  28. Rippka JR, Deruelles J, Waterbury JB, Herdmann M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

  29. Schaeffer F, Stanier RY (1978) Glucose-6-phosphate dehydrogenase of Anabaena sp. Kinetic and molecular properties. Arch Microbiol 116:9–19

  30. Scheibe P, Anderson LE (1981) Dark modulation of NADP-dependent malate dehydrogenase in the chloroplast. Biochim Biophys Acta 636:58–64

  31. Scholes P, Mitchell P, Moyle J (1969) The polarity of proton translocations in some photosynthetic micro-organisms. Eur J Biochem 8:450–454

  32. Stanier RY, Cohen-Bazire G (1977) Phototrophic procaryotes: the cyanobacteria. Annu Rev Microbiol 31:225–274

  33. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bact Rev 35:171–205

  34. Takahama U, Schimizu-Takahama M, Heber U (1981) The redox state of the NADP system in illuminated chloroplasts. Biochim Biophys Acta 637:530–539

  35. Veronese FM, Boccu E, Fontana A, Benassi CA, Scoffone E (1974) Isolation and some properties of 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus. Biochim Biophys Acta 334:31–44

Download references

Author information

Correspondence to Henk J. Lubberding.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lubberding, H.J., Bot, P.V.M. The influence of temperature on glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase and the regulation of these enzymes in a mesophilic and a thermophilic cyanobacterium. Arch Microbiol 137, 115–120 (1984).

Download citation

Key words

  • Cyanobacteria
  • Synechococcus
  • Synechocystis
  • Thermophily
  • Temperature effects
  • Glucose-6-phosphate dehydrogenase
  • 6-Phosphogluconate dehydrogenase
  • Enzyme kinetics
  • Enzyme regulation