Advertisement

Archives of Microbiology

, Volume 151, Issue 2, pp 101–104 | Cite as

Light-induced accumulation of lactate and succinate in Anabaena cylindrica

  • Masayuki Ohmori
  • Yasohachi Satoh
  • Katsuro Urata
Original Papers

Abstract

In the cyanobacterium Anabaena cylindrica lactate accumulated in large amounts when the cells were exposed to light. The presence or absence of oxygen, or a change in CO2 concentration did not affect the lactate accumulation. The cellular succinate level also increased in the light when CO2 was supplied at the high concentration of 1%. 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU), an inhibitor of photosynthetic electron flow, inhibited the increase in the concentration of lactate and succinate. Photosynthesis is a prerequisite for the increase of these organic acids. Thenoyltrifluoroacetone, an inhibitor of succinate dehydrogenase, inhibited the increase of succinate, suggesting that the succinate is formed via fumarate by the reverse of reactions of tricarboxylic acid (TCA) cycle. Upon addition of ammonium to the cell suspension in the light under high CO2 concentration, the increases in the concentrations of lactate and succinate were inhibited while those of glutamine, glutamate and aspartate were stimulated. Ammonium apparently changed the products of metabolism of pyruvate and oxaloacetate from lactate and succinate to amino acids.

Key words

Cyanobacteria Anabaena cylindrica Organic acid accumulation Ammonium effect 

Abbreviations

Chl

chlorophyll

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethyl urea

TTFA

thenoyltrifluoroacetone

PCA

perchloric acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Amarasingham CR, Davis BD (1965) Regulation of α-ketoglutarate dehydrogenase formation in Escherichia coli. J Biol Chem 240:3664–3667Google Scholar
  2. Baginsky ML, Hatefi Y (1969) Reconstitution of succinate-coenzyme Q reductase (complex II) and succinate oxidase activities by a highly purified, reactivated succinate dehydrogenase. J Biol Chem 244:5313–5319Google Scholar
  3. Biggins J (1969) Respiration in Blue-green algae. J Bacteriol 99:570–575Google Scholar
  4. Callely AG, Rigopoulos N, Fuller RC (1968) The assimilation of carbon by Chloropseudomonas ethylicum. Biochem J 106:615–622Google Scholar
  5. Houchins JP, Hind G (1982) Pyridine nucleotides and H2 as electron donors to the respiratory and photosynthetic electron-transfer chains and to nitrogenase in Anabaena heterocysts. Biochim Biophys Acta 682:86–96Google Scholar
  6. Kanazawa T, Kirk MR, Bassham JA (1970) Regulation of photosynthetic carbon metabolism in photosynthesizing Chlorella pyrenoidosa. Biochim Biophys Acta 205:401–408Google Scholar
  7. Kanazawa T, Kanazawa K, Kirk MR, Bassham JA (1972) Regulatory effects of ammonia on carbon metabolism in Chlorella pyrenoidosa during photosynthesis and respiration. Biochim Biophys Acta 226:656–669Google Scholar
  8. Kelly DP (1971) Autotrophy: Concepts of lithotrophic bacteria and their organic metabolism. Ann Rev Microbiol 25:177–210Google Scholar
  9. Lawric AC, Codd GA, Stewart WDP (1976) The incorporation of nitrogen into products of recent photosynthesis in Anabaena cylindrica Lemm. Arch Microbiol 107:15–24Google Scholar
  10. Leach CK, Carr NG (1969) Oxidative phosphorylation in an extract of Anabaena variabilis. Biochem J 112:125–126Google Scholar
  11. Leach CK, Car NG (1970) Electron transport and oxidative phosphorylation in the blue-green alga Anabaena cylindrica. J Gen Microbiol 64:55–70Google Scholar
  12. Lucas C, Weitzman PDJ (1977) Regulation of citrate synthase from blue-green bacteria by succinyl coenzyme A. Arch Microbiol 114:55–60Google Scholar
  13. Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322Google Scholar
  14. Matsumura H, Miyachi S (1980) Cycling assay for nicotinamide adenine dinucleotides. Meth Enzymol 69:465–470Google Scholar
  15. Ohmori M, Hattori A (1978) Transient change in the ATP pool of Anabaena cylindrica associated with ammonia assimilation. Arch Microbiol 117:17–20Google Scholar
  16. Ohmori M (1981) Effect of ammonia on dark CO2 fixation by Anabaena cells treated with methionine sulfoximine. Plant Cell Physiol 22:709–716Google Scholar
  17. Ohmori M, Miyachi S, Okabe K, Miyachi S (1984) Effects of ammonia on respiration, adenylate levels, amino acid synthesis and CO2 fixation in cells of Chlorella vulgaris 11 h in darkness. Plant Cell Physiol 25:749–756Google Scholar
  18. Pearce J, Carr NG (1967) An incomplete tricarboxylic acid cycle in the blue-green alga Anabaena variabilis. Biochem J 105:45Google Scholar
  19. Pearce J, Carr NG (1969) The incorporation and metabolism of glucose by Anabaena variabilis. J Gen Microbiol 54:451–462Google Scholar
  20. Pearce J, Leach CK, Carr NG (1969) The incomplete tricarboxylic acid cycle in the blue-green alga Anabaena variabilis. J Gen Microbiol 55:371–378Google Scholar
  21. Pelroy RA, Bassham JA (1972) Photosynthetic and dark carbon metabolism in unicellular blue-green algae. Arch Mikrobiol 86:25–38Google Scholar
  22. Pelroy RA, Rippka R, Stanier RY (1972) Metabolism of glucose by unicellular blue-green algae. Arch Mikrobiol 87:303–322Google Scholar
  23. Salerno JC, Ohnishi T (1980) Studies on the stabilized ubisemiquinone species in the succinate-cytochrome c reductase segment of the intact mitochondrial membrane system. Biochem J 192:769–781Google Scholar
  24. Schrautemeier B, Böhme H, Böger P (1985) Reconstitution of a light-dependent nitrogen-fixing and transhydrogenase system with heterocyst thylakoids. Biochim Biophys Acta 807:147–154Google Scholar
  25. Smith AJ, London J, Stanier RY (1967) Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli. J Bacteriol 94:972–983Google Scholar
  26. Van Baalen C, Hoare DS, Brandt S (1971) Heterotrophic growth of blue-green algae in dim light. J Bacteriol 105:685–689Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Masayuki Ohmori
    • 1
  • Yasohachi Satoh
    • 2
  • Katsuro Urata
    • 3
  1. 1.Ocean Research InstituteUniversity of TokyoTokyo 164Japan
  2. 2.Department of Biology, College of Arts and SciencesUniversity of TokyoTokyo 153Japan
  3. 3.Oyama National College of TechnologyOyama, Tochigi 323Japan

Personalised recommendations