Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Assimilatory sulfur metabolism in Rhodopseudomonas sulfoviridis

  • 36 Accesses

  • 13 Citations

Abstract

Rhodopseudomonas sulfoviridis is unable to grow with sulfate as sole sulfur source. Radioactively labelled sulfate is not incorporated into the cells. Growth only occurs in the presence of reduced sulfur compounds, such as sulfide, thiosulfate, elemental sulfur and cysteine. ATP sulfurylase, adenylylsulfate kinase, O-acetylserine sulfhydrylase and cysteine desulfhydrase are present. Adenylylsulfate sulfotransferase and thiosulfonate reductase are lacking. The enzymes of the sulfate-activating system are not derepressed by O-acetylserine.

This is a preview of subscription content, log in to check access.

Abbreviations

APS:

Adenosine 5′-phosphosulfate

PAPS:

3′-phosphoadenosine 5′-phosphosulfate

References

  1. Chambers LA, Trudinger PA (1971) Cysteine and S-sulfocysteine biosynthesis in bacteria. Arch Mikrobiol 77:165–184

  2. Cooper BP (1980) Der enzymatische Mechanismus der Sulfataktivierung bei Rhodospirillaceae. Doctoral Thesis, University of Bonn, FRG

  3. Hansen TA, Veldkamp H (1973) Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple nonsulfur bacteria. Arch Mikrobiol 92:45–58

  4. Hansen TA (1974) Sulfide als electrondonor voor Rhodospirillaceae. Doctoral Thesis, University of Groningen, Holland

  5. Imhoff JF (1980) Aspekte des assimilatorischen Schwefelstoffwechsels in Rhodospirillaceae. Doctoral Thesis, University of Bonn, FRG

  6. Imhoff JF, Then J, Hashwa F, Trüper HG (1981) Sulfate assimilation in Rhodopseudomonas globiformis. Arch Microbiol 130:234–237

  7. Imhoff JF, Trüper HG (1977) Ectothiorhodospira halochloris, sp. nov., a new extremely halophilic phototrophic bacteria containing bacteriochlorophyll b. Arch Microbiol 114:115–121

  8. Keppen OI, Gorlenko VM (1975) A new species of purple budding bacteria containing bacteriochlorophyll b. Mikrobiologiya 44:258–263

  9. Kumagai H, Sejima S, Choi YJ, Tanaka H, Yamada H (1975) Crystallization and properties of cysteine desulfhydrase from Aerobacter aerogenes. FEBS Lett 52:304–307

  10. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275

  11. Pachmayr F (1966) Vorkommen und Bestimmung von Schwefelverbindungen in Mineralwasser. Doctoral Thesis, University of München, FRG

  12. Pfennig N (1974) Rhodopseudomonas globiformis, sp. n., a new species of the Rhodospirillaceae. Arch Microbiol 100:197–206

  13. Roy AB, Trudinger PA (1970) The biochemistry of inorganic compounds of sulfur. Cambridge University Press: 43–44

  14. Schmidt A (1972) On the mechanism of photosynthetic sulfate reduction. An APS-sulfotransferase from Chlorella. Arch Mikrobiol 84:77–86

  15. Schmidt A (1973) Sulfate reduction in a protein-bound intermediate by a thiosulfonate reductase. Arch Mikrobiol 93:29–52

  16. Siegel LM (1975) Biochemistry of the sulfur cycle. In: Greenberg DM (ed) Metabolic pathways, VII. Metabolism of sulfur compounds. Academic, New York, pp 217–286

  17. Stickland LH (1951) The determination of small quantities of bacteria by means of the biuret reaction. J Gen Microbiol 5:698–703

  18. Wilson LG, Bandurski RS (1958) Enzymatic reactions involving sulfate, sulfite, selenate and molybdate. J Biol Chem 233:975–981

Download references

Author information

Correspondence to Otmar Neutzling.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Neutzling, O., Trüper, H.G. Assimilatory sulfur metabolism in Rhodopseudomonas sulfoviridis . Arch. Microbiol. 133, 145–148 (1982). https://doi.org/10.1007/BF00413529

Download citation

Key words

  • Rhodopseudomonas sulfoviridis
  • Assimilatory sulfur metabolism
  • Sulfur assimilation