Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Electron transport and respiration in Beggiatoa and Vitreoscilla

Abstract

Seven strains of bacteria belonging to the Beggiatoa-Vitreoscilla group were studied for their respiratory activity and for the presence of electron transport conponents. All strains tested oxidized [1-14C] and [2-14C] acetate to 14CO2 at relatively high rates. All strains tested were N,N,N′,N′-tetramethylphenylenediamine (TMPD)-oxidase positive and contained spectra representing a-type and carbon monoxide-binding cytochromes. Most of the strains also contained spectra representing c-type and b-type cytochromes. Beggiatoa alba B18LD contained b-type, a-type, c-type and CO-binding cytochromes, the latter two being located in the 144,000 x g soluble fraction. B. alba also contained ubiquinone-8 as its only detectable quinone.

This is a preview of subscription content, log in to check access.

Abbreviations

BSS:

basal salts solution

BH:

Beggiatoa heterotrophic medium

BSO:

Beggiatoa sulfide oxidation medium

TMPD:

N,N,N′,N′-tetramethylphenylenediamine

Q8:

ubiquinone-8

References

  1. Burton SD, Morita RY (1964) Effect of catalase and cultural conditions on growth of Beggiatoa. J Bacteriol 88:1755–1761

  2. Burton SD, Morita RY, Miller M (1966) Utilization of acetate by Beggiatoa. J Bacteriol 91:1192–1200

  3. Cannon GC, Strohl WR, Larkin JM, Shively JM (1979) Cytochromes in Beggiatoa alba. Curr Microbiol 2:263–266

  4. Carr NG, Exell G, Flynn V, Hallaway M, Talukdar S (1967) Minor quinones of some Myxophyceae. Arch Biochem Biophys 120:503–507

  5. DiSpirito AA, Loh W H-T, Tuovinen OH (1983) A novel method for the isolation of bacterial quinones and its application to appraise the ubiquinone composition of Thiobacillus ferrooxidans. Arch Microbiol 135:77–80

  6. Güde H, Strohl WR, Larkin JM (1981) Mixotrophic and heterotrophic growth of Beggiatoa alba in continuous culture. Arch Microbiol 129:357–360

  7. Kelly DP (1982) Biochemistry of the chemolithotrophic oxidation of inorganic sulphur. Phil Trans R Soc Lond B 298:499–528

  8. Kowallik U, Pringsheim EG (1966) The oxidation of hydrogen sulfide by Beggiatoa. Am J Bot 53:801–806

  9. Kuenen JG, Beudecker RF (1982) Microbiology of thiobacilli and other sulphur-oxidizing autotrophs, mixotrophs and heterotrophs. Phil Trans R Soc Lond B 298:473–497

  10. Larkin JM, Strohl WR (1983) Beggiatoa, Thiothrix, and Thioploca. Annu Rev Microbiol 37:341–367

  11. Mayfield DC, Kester AS (1975) Nutrition of Vitreoscilla stercoraria. Can J Microbiol 21:1947–1951

  12. Mezzino MJ, Strohl WR, Larkin JM (1984) Characterization of Beggiatoa alba. Arch Microbiol 137:139–144

  13. Minges CG, Titus JA, Strohl WR (1983) Plasmid DNA in colorless filamentous gliding bacteria. Arch Microbiol 134:38–44

  14. Nelson DC, Castenholz RW (1981) Use of reduced sulfur compounds by Beggiatoa sp. J Bacteriol 1947:140–154

  15. Nelson DC, Jannasch HW (1983) Chemoautotrophic growth of a marine Beggiatoa in sulfide-gradient cultures. Arch Microbiol 136:262–269

  16. Pringsheim EG (1964) Heterotrophism and species concepts in Beggiatoa. Am J Bot 51:893–913

  17. Pringsheim EG (1967) Die Mixotrophie von Beggiatoa. Arch Mikrobiol 59:247–254

  18. Schmidt TM, Vinci VA, Strohl WR (1986) Protein synthesis by Beggiatoa alba in the presence and absence of sulfide. Arch Microbiol 144:158–162

  19. Scotten HL, Stokes JL (1962) Isolation and properties of Beggiatoa. Arck Mikrobiol 42:353–368

  20. Strohl WR, Larkin JM (1978) Enumeration, isolation, and characterization of Beggiatoa from freshwater sediments. Appl Environ Microbiol 36:755–770

  21. Strohl WR, Schmidt TM (1984) Mixotrophy in Beggiatoa and Thiothrix. In: Strohl WR, Tuovinen OH (eds) Microbial chemoautotrophy. The Ohio State University Press, Columbus, pp 79–95

  22. Strohl WR, Cannon GC, Shively JM, Güde H, Hook LA, Lane CH, Larkin JM (1981) Heterotrophic carbon metabolism by Beggiatoa alba. J Bacteriol 148:572–583

  23. Strohl WR, Schmidt TM, Lawry NH, Mezzino MJ, Larkin JM (1986) Characterization of Vitreoscilla beggiatoides and V. filiformis sp. nov., nom. rev., and comparison to V. stercoraria and Beggiatoa. Internat J Syst Bacteriol (in press)

  24. Vargas A, Strohl WR (1985a) Ammonium assimilation by Beggiatoa alba. Arch Microbiol 142:275–278

  25. Vargas A, Strohl WR (1985b) Utilization of, nitrate by Beggiatoa alba. Arch Microbiol 142:279–284

  26. Webster DA, Hackett P (1966) Respiratory chain of colorless algae. II. Cyanophyta. Plant Physiol 41:599–605

Download references

Author information

Correspondence to William R. Strohl.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Strohl, W.R., Schmidt, T.M., Vinci, V.A. et al. Electron transport and respiration in Beggiatoa and Vitreoscilla . Arch. Microbiol. 145, 71–75 (1986). https://doi.org/10.1007/BF00413029

Download citation

Key words

  • Beggiatoa
  • Vitreoscilla
  • Cytochromes
  • Electron transport
  • Sulfide oxidation
  • Acetate oxidation
  • TMPD oxidase