Occurrence of Curtobacterium sp. possessing ω-cyclohexyl fatty acids in soil with zinc added
A bacterial strain, Curtobacterium sp., isolated from a soil with zinc added possessed ω-cyclohexyl fatty acids. ω-Cyclohexyl undecanoic acid made up 47% of the total fatty acids; it was the most abundant fatty acid in the strain grown in tryptone medium. 12-Methyl tetradecanoic acid (23%) and 14-methyl hexadecanoic acid (22%) were also major fatty acids. The proportion of ω-cyclohexyl undecanoic acid increased as the pH of the medium decreased and as the culture temperature increased.
The bacteria grew almost normally in zinc-enriched medium, and ω-cyclohexyl undecanoic acid increased with zinc concentration. Zinc added to the medium was not abundant in the cell fraction, and the ratio of increase of zinc in the cells was not so high as in the culture medium. These results suggested that ω-cyclohexyl fatty acids are related to the zinc tolerance of the isolated strain, and that this tolerance depends on low permeability of the membrane to zinc.
Key wordsω-Cyclohexyl undecanoic acid Zinc-tolerant bacteria Fatty acid composition Cyclohexyl fatty acid Bacillus acidocaldarius Curtobacterium pusillum Curtobacterium sp.
Unable to display preview. Download preview PDF.
- Darland G, Brock TD (1971) Bacillus acidocaldarius sp.nov., an acidophilic thermophilic spore-forming bacterium. J Gen Microbiol 67: 9–15Google Scholar
- De Rosa M, Gambacorta A, Minale L, Bu'Lock JD (1971) Bacterial triterpenes. Chem Commun 619–620Google Scholar
- De Rosa M, Gambacorta A, Minale L, Bu'Lock JD (1972) The formation of ω-cyclohexyl-fatty acids from shikimate in an acidophilic thermophilic Bacillus: A new biosynthetic pathway. Biochem J 128: 751–754Google Scholar
- De Rosa M, Gambacorta A, Bu'Lock JD (1974) Effects of pH and temperature on the fatty acid composition of Bacillus acidocaldarius. J Bacteriol 117: 212–214Google Scholar
- Duxbury T (1981) Toxicity of heavy metals to soil bacteria. FEMS Microbiol Lett 11: 217–220Google Scholar
- Duxbury T, Bicknell B (1983) Metal-tolerant bacterial populations from natural and metal-polluted soils. Soil Biol Biochem 15: 243–250Google Scholar
- Hansen RP (1967) 11-Cyclohexylundecanoic acid: Its occurrence in bovine rumen bacteria. Chem Ind 1640–1641Google Scholar
- Hippchen B, Röll A, Poralla K (1981) Occurrence in soil of thermoacidophilic bacilli possessing ω-cyclohexane fatty acids and hopanoids. Arch Microbiol 129: 53–55Google Scholar
- Iizuka H, Komagata K (1965) Determination of Brevibacterium, Arthrobacter, Micrococcus, Sarcina, Alcaligenes and Achromobacter isolated from oil-brines in Japan. J Gen Appl Microbiol 11: 1–14Google Scholar
- Jordan MJ, Lechevalier MP (1975) Effects of zinc-smelter emissions on forest soil microflora. Can J Microbiol 21: 1855–1865Google Scholar
- Lennarz WJ (1966) Lipid metabolism in the bacteria. Adv Lipid Res 4: 175–225Google Scholar
- Ohya H, Komai Y, Yamaguchi M (1985)Zine effects on soil microflora and glucose metabolites in soil amended with 14C-glucose. Biol Fertil Soils 1: 117–122Google Scholar
- Ohya H, Komai Y, Yamaguchi M (1986) Zinc tolerance of an isolated bacterium containing ω-cyclohexyl fatty acid. FEMS Microbiol Lett (in press)Google Scholar
- Oshima M, Ariga T (1975) ω-Cyclohexyl fatty acids in a acidophilic thermophilic bacteria. J Biol Chem 250: 6963–6968Google Scholar
- Peterson SL, Bennett LG, Tornabene TG (1975) Effects of lead on the lipid composition of Micrococcus luteus cells. Appl Microbiol 29: 669–679Google Scholar
- Poralla K, König WA (1983) The occurrence of ω-cycloheptane fatty acids in a thermo-acidophilic bacillus. FEMS Microbiol Lett 16: 303–306Google Scholar
- Schogt JC, Begemann PH (1965) Isolation of 11-cyclohexyl-undecanoic acid from butter. J Lipid Res 6: 466–470Google Scholar
- Sterritt RM, Lester JN (1980) Interactions of heavy metals with bacteria. Sci Total Environ 14: 5–17Google Scholar
- Suzuki K, Saito K, Kawaguchi A, Okuda S, Komagata K (1981) Occurrence of ω-cyclohexyl fatty acids in Curtobacterium pusillum strains. J Appl Microbiol 27: 261–266Google Scholar
- Tornabene TG, Edwards HW (1972) Microbial uptake of lead. Science 176: 1334–1335Google Scholar
- Tornabene TG, Edwards HW (1973) Effects of lead on bacterial membranes. In: Hemphill DD (ed) Trace substances in environmental health, vol 7. University of Missouri Press, Columbia, pp 263–266Google Scholar