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Ultra-violet induced mutations to growth factor requirement and penicillin resistance in a blue-green alga

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Summary

After exposures to UV, two mutant strains of Phormidium mucicola were isolated which are stable and inheritable:

1. Strain 5/5 mL-1 is a photoheterotrophic mutant requiring addition of an organic growth factor to the basal medium which appears fulfilled by either casein hydrolysate, glucose, acetate or vitamin mixture and fails to grow heterotrophically.

2. mr/p. This strain shows resistance upto 10.24 μg/ml of penicillin whereas the parent does not survive beyond a penicillin dose of 0.1 μg/ml.

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References

  1. Allen, M. B., and D. I. Arnon: Studies on nitrogen fixing blue-green alga. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiol. 30, 366–372 (1955).

  2. Beadle, G. W., and E. L. Tatum: Genetic control of biochemical reactions in Neurospora. Proc. nat. Acad. Sci. (Wash.) 27, 499–506 (1941).

  3. Bonner, D. M., C. Yanofsky, and C. W. Partridge: Incomplete genetic blocks in biochemical mutants of Neurospora. Proc. nat. Acad. Sci. (Wash.) 38, 25 (1952).

  4. Rowley, D.: Selection of penicilin-sensitive mutants of Escherichia coli following ultraviolet irradiation. J. gen. Microbiol. 6, 272–277 (1952).

  5. Singh, R. N., and H. N. Singh: Ultraviolet induced mutants of blue-green algae. I. Anabaena cycadeae Reinke. Arch. Mikrobiol. 48, 109–117 (1964a).

  6. ——: Ultra-violet induced mutants of blue-green algae. II. Anabaena doliolum Bharadwaja. Arch. Mikrobiol. 48, 117–121 (1964b).

  7. Srivastava, B. S., and H. D. Kumar: Pigment variation in ultra-violet treated strains of the blue-green alga Anabaena doliolum. In: The role of genetics today. Intern. Symp., Hyderabad, 2.–4. September 1968 (in press).

  8. Strauss, B. S., and T. Minagawa: Formation of methionine by a methionine requiring mutant of Neurospora crassa. J. gen. Microbiol. 20, 237–245 (1959).

  9. Witkin, E. M.: Genetics of resistance to radiation in Escherichia coli. Genetics 32, 221–248 (1947).

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Srivastava, B.S. Ultra-violet induced mutations to growth factor requirement and penicillin resistance in a blue-green alga. Archiv. Mikrobiol. 66, 234–238 (1969). https://doi.org/10.1007/BF00412055

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Keywords

  • Glucose
  • Acetate
  • Growth Factor
  • Penicillin
  • Basal Medium