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Enzymologic and histologic investigations in normal and pressure-ischemic retina of rabbits

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Histologic investigations together with histochemical and photometric measurements of enzyme activities were performed in retina of rabbits, whose blood supply had been totally interrupted for 1h. A retinal edema developed affecting the internal layers between the inner limiting membrane and the internal plexiform and ganglion cell layer. Although this edema was quite remarkable at the posterior pole of the eye, it diminished toward the periphery, disappearing near the ora serrata.

The activities of the following enzymes were investigated: hexokinase, glucose 6-phosphate dehydrogenase, aldolase, glyceraldehydephosphate dehydrogenase, lactate dehydrogenase, malate dehydrogenase, succinate dehydrogenase, ATPase, and phosphorylase. The most striking finding was the total disappearance of phosphorylase activity under pressure ischemia. ATPase and aldolase showed a decreased activity in the ischemic retina, and malate dehydrogenase a slightly diminished one. Concerning the other enzymes, no significant differences between normal and ischemic retina were observed.


An Kaninchen-Netzhäuten, deren Durchblutung für die Dauer einer Stunde völlig unterbrochen worden war, wurden histologische Untersuchungen sowie histochemische und spektralphotometrische Enzymaktivitätsbestimmunge durchgeführt. Es entwickelte sich ein retinales Ödem in den inneren Schichten zwischen der inneren Grenzmembran und der inneren plexiformen und Ganglienzellschicht. Das Ödem war am hinteren Augenpol ziemlich ausgeprägt, verminderte sich jedoch zur Peripherie hin und verschwand völlig nahe der Ora serrata. Die Aktivität folgender Enzyme wurde untersucht: Hexokinase, Glukose-6-Phosphatdehydrogenase, Aldolase, Glycerinaldehydphosphatdehydrogenase, Laktatdehydrogenase, Malatdehydrogenase, Succinatdehydrogenase, ATPase und Phosphorylase. Das auffallendste Ergebnis war das völlige Verschwinden der Phosphorylaseaktivität unter Druckischaemie. ATPase und Aldolase zeigten eine verminderte Aktivität und Malatdehydrogenase eine leicht verringerte Aktivität in der ischaemischen Retina. Alle anderen Enzyme ließen keine signifikanten Unterschiede zwischen normaler und ischaemischer Netzhaut erkennen.

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  1. Bergmeyer, H.-U.: Methoden der enzymatischen Analyse. 2. Aufl. Vol. II, p. 1163; 1172; 1200; 1266, Weinheim: Verl. Chemie, 1970

  2. Forgacs, J., Babel, J.: L'activité déhydrogenasique dans l'ischémie expérimentale de la rétine du lapin. Bull. Soc. belge Ophthal. 136, 243–251 (1964)

  3. Kaskel, D., Hockwin, O., Metzler, U., Schedtler, C.-M.: Glycogen content in the rabbit retina in relation to blood circulation. Ophthal. Res. 5, 177–185 (1973)

  4. Kuwabara, T., Cogan, D.G.: Retinal glycogen. Arch. Ophthal. 66, 680–688 (1961)

  5. Nachlas, M.M. and Schnitka, T.S.: Macroscopic identification of early myocardial infarcts by alterations in dehydrogenase activity. Amer. J. Path. 42, 379–405 (1963)

  6. Oberhoff, P., Hockwin, O.: The ATP content of the retina in relation to the blood circulation. Graefes Arch. Ophthal. 178, 329–332 (1969)

  7. Scholz, R., Kaskel, D., Hockwin, O.: The effect of unilateral pressure-ischaemia on the carbohydrate metabolism in ocular tissues. Ophthal. Res. (in print)

  8. Schulze, W., Krause, E.-G., Wollenberger, A.: On the fate of glycogen phosphorylase in the ischaemic and infarcting myocardium. J. Molecular Cell. Card. 2, 231–251 (1971)

  9. Vassileva, P.: Studies on some dehydrogenases in experimental retinal ischaemia. Ophthal. Res. 7, 381–389 (1975)

  10. Weichselbaum, T.E.: An accurate and rapid method for the determination of proteins in small amounts of blood serum and plasma. Amer. J. Clin. Path. Suppl. 10, 40–49 (1946)

  11. Weiss, H.: The carbohydrate reserve in the vitreous body and retina of the rabbit's eye during and after pressure-ischaemia and insulin hypoglycaemia. Ophthal. Res. 3, 360–371 (1972)

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Fellow of the Alexander von Humboldt-Stiftung.

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Kaskel, D., Valenzuela, H., Hockwin, O. et al. Enzymologic and histologic investigations in normal and pressure-ischemic retina of rabbits. Albrecht v. Graefes Arch. klin. exp. Ophthal. 200, 71–78 (1976). https://doi.org/10.1007/BF00411434

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  • Retina
  • Phosphorylase
  • Hexokinase
  • Succinate Dehydrogenase
  • Aldolase