Archives of Microbiology

, Volume 131, Issue 4, pp 351–355 | Cite as

Production of an aromatic aldehyde oxidase by Streptomyces viridosporus

  • Don L. Crawford
  • John B. Sutherland
  • Anthony L. PomettoIII
  • Jody M. Miller
Original Papers


Streptomyces viridosporus strain T7A, when grown in liquid media containing yeast extract and aromatic aldehydes, oxidized the aromatic aldehydes to the corresponding aromatic acids. Benzaldehyde, m-hydroxybenzaldehyde, p-hydroxybenzaldehyde, and protocatechualdehyde were catabolized further via the β-ketoadipate and gentisate pathways. Dehydrodivanillin, isophthalaldehyde, salicylaldehyde, syringaldehyde, terephthalaldehyde, vanillin, and veratraldehyde were oxidized only as far as the corresponding aromatic acids. Phthalaldehyde and aliphatic aldehydes were not oxidized. The aromatic aldehyde oxidase, which was produced by cultures grown in either the presence or absence of aromatic aldehydes, was partially purified by ammonium sulfate precipitation and ion-exchange chromatography. It consumed molecular oxygen, oxidized aromatic aldehydes to aromatic acids, and produced hydrogen peroxide all in equimolar amounts.

Key words

Aldehyde oxidase Aromatic aldehydes Streptomyces viridosporus 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Booth H, Saunders BC (1956) Studies in peroxidase action. Part X. The oxidation of phenols. J Chem Soc 1956:940–948Google Scholar
  2. Crawford RL, Crawford DL, Dizikes GJ (1981) Catabolism of the lignin substructure model compound dehydrodivanillin by a lignin-degrading Streptomyces. Arch Microbiol 129:204–209Google Scholar
  3. Davey JF, Gibson DT (1974) Bacterial metabolism of para- and meta-xylene oxidation of a methyl substituent. J Bacteriol 119:923–929Google Scholar
  4. Eggeling L, Sahm H (1980) Degradation of coniferyl alcohol and other lignin-related aromatic compounds by Nocardia sp. DSM 1069. Arch Microbiol 126:141–148Google Scholar
  5. Elbs K, Lerch H (1916) Über Dehydrodivanillin. J Prakt Chem 93:1–9Google Scholar
  6. Gordon AH, Green DE, Subrahmanyan V (1940) Liver aldehyde oxidase. Biochem J 34:764–774Google Scholar
  7. Hopper DJ, Kemp PD (1980) Regulation of enzymes of the 3,5-xylenol-degradative pathway in Pseudomonas putida: evidence for a plasmid. J Bacteriol 142:21–26Google Scholar
  8. Katayama Y, Fukuzumi T (1979) Bacterial degradation of dimers structurally related to lignin. III. Metabolism of α-veratryl-β-guaiacylpropionic acid and D,l-pinoresinol by Pseudomonas putida. Mokuzai Gakkaishi 25:67–76Google Scholar
  9. Keat MJ, Hopper DJ (1975) Aromatic aldehyde dehydrogenase from Pseudomonas putida N.C.1.B. 9869. Biochem Soc Trans 3:385–386Google Scholar
  10. Kingsley GR, Getchell G (1960) Direct ultramicro glucose oxidase method for determination of glucose in biologic fluids. Clin Chem 6:466–475Google Scholar
  11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  12. Muranaka M, Kinoshita S, Yamada Y, Okada H (1976) [Decomposition of lignin model compound, 3-(2-methoxy-4-formylphenoxy)-1,2-propanediol by bacteria.] J Ferment Technol 54:635–639 (in Japanese)Google Scholar
  13. Pometto AL, Sutherland JB, Crawford DL (1981) Streptomyces setonii: catabolism of vanillic acid via guaiacol and catechol. Can J Microbiol 27:636–638Google Scholar
  14. Robbins WJ, Lathrop EC (1919) The oxidation of vanillin to vanillic acid by certain soil bacteria. Soil Sci 7:475–485Google Scholar
  15. Stanier RY (1948) The oxidation of aromatic compounds by fluorescent pseudomonads. J Bacteriol 55:477–494Google Scholar
  16. Sutherland JB, Crawford DL, Pometto AL (1981) Catabolism of substituted benzoic acids by Streptomyces species. Appl Environ Microbiol 41:442–448Google Scholar
  17. Tadasa K (1977) Degradation of eugenol by a microorganism. Agric Biol Chem 41:925–929Google Scholar
  18. Toms A, Wood JM (1970) The degradation of rans-ferulic acid by Pseudomonas acidovorans. Biochemistry 9:337–343Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Don L. Crawford
    • 1
  • John B. Sutherland
    • 1
  • Anthony L. PomettoIII
    • 1
  • Jody M. Miller
    • 1
  1. 1.Department of Bacteriology and Biochemistry, Idaho Agricultural Experiment StationUniversity of IdahoMoscowUSA

Personalised recommendations