Advertisement

Archives of Microbiology

, Volume 130, Issue 2, pp 93–95 | Cite as

Possible role of membrane proteins in mercury resistance of Enterobacter aerogenes

  • Hidemitsu S Pan-Hou
  • Masayo Nishimoto
  • Nobumasa Imura
Article

Abstract

Mercury resistance shown by a strain of Enterobacter aerogenes was found to be determined by a plasmid. The resistance appeared to be not due to enzymatic volatilization of mercury, but due to the alteration in cellular permeability to mercury.

Comparison of the outer membrane proteins was made between the resistant cells and the sensitive counterparts obtained by the treatment with mitomycin C, showing that two proteins with molecular weight of 46,000 and 44,000 had disappeared from the outer membrane along with the plasmid by the curing. These results suggest that the two membrane proteins mediating the cellular permeability to mercury compound may be responsible for the mercury resistance of the strain.

Key words

Membrane proteins Mercury resistance Plasmid-mediated Uptake of mercury Permeability Outer membrane Enterobacter aerogenes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chopra I (1971) Decreased uptake of cadmium by a resistant strain of Staphylococcus aureus. J Gen Microbiol 63:265–267Google Scholar
  2. Clark DK, Weiss AA, Silver S (1977) Mercury and organomercurial resistance determined by plasmid in Pseudomonas. J Bacteriol 132:186–196Google Scholar
  3. Greenaway W (1972) Permeability of phenyl-Hg+-resistant and phenyl-Hg+-susceptible isolates of Pyrenophore avenae to the phenyl-Hg+ ion. J Gen Microbiol 73:251–255Google Scholar
  4. Hansen JB, Olsen RH (1978) Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids PMG1 and PMG5. J Bacteriol 135:227–238Google Scholar
  5. Horitsu H, Ito T (1980) Comparisons of characteristics of mercury tolerant bacterium Pseudomonas oleovorans G-1 and its mercury sensitive mutant strain. Agric Biol Chem 44:2317–2322Google Scholar
  6. Ishihara M, Kamio Y, Terawaki Y (1978) Cupric ion resistance as a new genetic marker of a temperature sensitive R plasmid, Rtsl in Escherichia coli. Biochem Biophys Res Commun 82:74–80Google Scholar
  7. Komura I, Izaki K (1971) Mechanism of mercuric chloride resistance in microorganisms. 1. Vaporization of a mercury compound from mercuric chloride by multiple drug resistance strain of Escherichia coli. J Biochem 70:885–893Google Scholar
  8. Kondo I, Ishikawa T, Nakahara H (1974) Mercury and cadmium resistance mediated by the penicillinase plasmid in Staphylococcus aureus. J Bacteriol 117:1–7Google Scholar
  9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685Google Scholar
  10. Lutkenhaus JF (1977) Role of a major outer membrane protein in Escherichia coli. J Bacteriol 131:631–637Google Scholar
  11. Manning PA, Beutin L, Achtman M (1980) Outer membrane of Escherichia coli. Properties of the F sex factor tra T protein which is involved in surface exclusion. J Bacteriol 142:285–294Google Scholar
  12. Miura T, Mizushima S (1969) Separation and properties of outer and cytoplasmic membranes in Escherichia coli. Biochim Biophys Acta 193:268–276Google Scholar
  13. Pan-Hou HSK, Imura N (1981) Role of hydrogen sulfide in mercury resistance determined by plasmid of Clostridium cochlearium T-2. Arch Microbiol 129:49–52Google Scholar
  14. Schnaitman CA (1971) Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. J Bacteriol 108:545–552Google Scholar
  15. Schottel J, Mandal A, Clark D, Silver S (1974) Volatilization of mercury and organomercurials determined by inducible R-factor in enteric bacteria. Nature 251:335–337Google Scholar
  16. Schottel JL (1978) The mercuric and organomercurial detoxifying enzymes from a plasmid-bearing strain of Escherichia coli. J Biol Chem 253:4341–4349Google Scholar
  17. Summers AO, Silver S (1972) Mercury resistance in a plasmid-bearing strain of Escherichia coli. 112:1228–1236Google Scholar
  18. Summers AO, Silver S (1978) Microbial transformations of metals. Ann Rev Microbiol 32:637–672Google Scholar
  19. Wang PY, Relf J, Palchaudhuri S, Iyer VN (1978) Plasmid coferring increased sensitivity to mercuric chloride and cobalt chloride found in some laboratory strain of Escherichia coli K-12. J Bacteriol 133:1042–1043Google Scholar
  20. Weiss AA, Murphy SD, Silver S (1977) Mercury and organomercurial resistance determined by plasmid in Staphylococcus aureus. J Bacteriol 132:197–208Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Hidemitsu S Pan-Hou
    • 1
  • Masayo Nishimoto
    • 1
  • Nobumasa Imura
    • 1
  1. 1.School of Pharmaceutical SciencesKitasato UniversityTokyoJapan

Personalised recommendations