Escherichia coli strain O 127: K63: (B8): H—was grown in nutrient broth (Difco). Penicillinase activity was found in the culture supernatant after only five hours of incubation, i.e. during the exponential phase of growth. At this phase the levels of typical intracellular “markers”, did not indicate cell lysis or gross cell damage. The bioautographic revelation of penicillin-splitting enzymes on electropherograms of cell-free liquids confirmed the presence of one basic broad-spectrum β-lactamase. This possibly extracellular β-lactamase seems to be also present in the cellular extracts where it coexists with several other cell-bound penicillinases.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Arcos, J. M., Chordi, A.: Séparation électrophorétique de β-lactam-hydrolases de Bacillus cereus. Ann. Inst. Pasteur 115, 1008–1017 (1968a).
— Ruiz, C., Múgica, J. D.: Coexistence of β-lactamases and acylases in Escherichia coli. J. Bact. 96, 1870–1872 (1968b).
Citri, N., Pollock, M. R.: The biochemistry and function of β-lactamase (penicillinase). Advanc. Enzymol. 28, 237–323 (1966).
Collins, C. H.: Microbiological methods. London: Butterworths 1967.
Dische, Z.: Color reactions of nucleic acid components. In: The nucleic acids, Vol. I, p. 227. Eds. E. Chargaff and J. N. Davison. New York: Academic Press 1955.
Meynell, G. G., Meynell, E.: Theory and practice in experimental bacteriology. Cambridge: University Press 1965.
Percival, A., Brumfitt, W., De Louvois, J.: The role of penicillinase in determining natural and acquired resistance in Gram-negative bacteria to penicillins. J. gen. Microbiol. 32, 77–89 (1963).
Pollock, M. R.: Exoenzymes. In: The bacteria, Vol. IV, p. 121. Eds. I. C. Gunsalus and R. Y. Stanier. New York: Academic Press 1962.
Rogers, H. J.: The dissimilation of high molecular weight substances. In: The bacteria, Vol. II, p. 257. Eds. I. C. Gunsalus and R. Y. Stanier. New York: Academic Press 1961.
Slein, M. W., Logan, G. F.: Lysis of Escherichia coli by ethylenediamine tetraacetate and phospholipases as measured by β-galactosidase activity. J. Bact. 94, 934–941 (1967).
Smith, J. T.: Penicillinase and ampicillin resistance in a strain of Escherichia coli. J. gen. Microbiol. 30, 299–306 (1963).
—, Hamilton-Miller, J. M. T.: Differences between penicillinases from Grampositive and Gram-negative sources. Nature (Lond.) 197, 976–978 (1963).
Weidel, W., Pelzer, H.: Bagshaped macromolecules: A new outlook on bacterial cell walls. Advanc. Enzymol. 26, 193–232 (1964).
About this article
Cite this article
Tanco, J.M., Miravalles, M.d.P. & Arcos, J.M. Extracellular β-lactamase from Escherichia coli . Archiv. Mikrobiol. 72, 16–22 (1970). https://doi.org/10.1007/BF00411010
- Escherichia Coli
- Culture Supernatant
- Cell Damage
- Exponential Phase