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Extracellular β-lactamase from Escherichia coli

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Escherichia coli strain O 127: K63: (B8): H—was grown in nutrient broth (Difco). Penicillinase activity was found in the culture supernatant after only five hours of incubation, i.e. during the exponential phase of growth. At this phase the levels of typical intracellular “markers”, did not indicate cell lysis or gross cell damage. The bioautographic revelation of penicillin-splitting enzymes on electropherograms of cell-free liquids confirmed the presence of one basic broad-spectrum β-lactamase. This possibly extracellular β-lactamase seems to be also present in the cellular extracts where it coexists with several other cell-bound penicillinases.

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Tanco, J.M., Miravalles, M.d.P. & Arcos, J.M. Extracellular β-lactamase from Escherichia coli . Archiv. Mikrobiol. 72, 16–22 (1970).

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  • Enzyme
  • Escherichia Coli
  • Culture Supernatant
  • Cell Damage
  • Exponential Phase