Archives of Microbiology

, Volume 146, Issue 4, pp 358–361 | Cite as

Energetics of glucose uptake in Salmonella typhimurium

  • M. Driessen
  • P. W. Postma
  • K. van Dam
Original Papers


We have studied the energetics of glucose uptake in Salmonella typhimurium. Strain PP418 transprots glucose via the phosphoenolpyruvate: glucose phosphotransferase system, while strain PP1705 lacks this system and can only use the galactose permease for glucose uptake. These two strains were cultured anaerobically in glucose-limited chemostats. Both strains produced ethanol and acetate in equimolar amounts but a significant difference was observed in the molar growth yield on glucose (YGlc). It is suggested that this difference is due to a difference in the energetics of the glucose uptake systems in the two strains.

Assuming an equal YATP for both strains, we could calculate that uptake of 1 mole of glucose via the galactose permease consumes the equivalent of 0.5 mole of ATP. With the additional assumption that one proton is transported in symport with one glucose molecule, these results imply a stoichiometry of two protons per ATP hydrolysed.

Key words

Growth yield Energetics (of growth) Salmonella typhimurium Glucose uptake 



Phosphoenolpyruvate: carbohydrate phosphotransferase system


dilution rate (h-1


dry weight


galactose permease














yield of cells per glucose or ATP


specific production rate


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bauchop T, Elsden SR (1960) The growth of microorganisms in relation to their energy supply. J Gen Microbiol 23:457–469Google Scholar
  2. Evans CTG, Herbert D, Tempest DW (1970) The continuous cultivation of micro-organisms. 2. Construction of a chemostat. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 2. Academic Press, New York, pp 277–327Google Scholar
  3. Gottschalk G (1979) Bacterial fermentations. In: Starr MP (ed) Bacterial metabolism Springer, Berlin Heidelberg New York, pp 167–224Google Scholar
  4. Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 5B. Academic Press New York, pp 209–344Google Scholar
  5. Hernandez E, Johnson MJ (1967) Anaerobic growth yields of Aerobacter cloacae and Escherichia coli. J Bacteriol 94:991–995Google Scholar
  6. Muir M, Williams L, Ferenci T (1985) Influence of transport energetization on the growth yield of Escherichia coli. J Bacteriol 163:1237–1242Google Scholar
  7. Nagelkerke F, Postma PW (1978) 2-Deoxygalactose, a specific substrate of the Salmonella typhimurium galactose permease: its use for the isolation of galP mutants. J Bacteriol 133:607–613Google Scholar
  8. Perlin SP, San Francisco MJD, Slayman CW, Rosen BP (1986) H+/ATP stoichiometry of proton pumps from Neurospora crassa and Escherichia coli. Arch Biochem Biophys 248:53–61Google Scholar
  9. Postma PW (1977) Galactose transport in Salmonella typhimurium. J Bacteriol 129:630–639Google Scholar
  10. Postma PW, Lengeler JW (1985) Phosphoenolpyruvate: carbohydrate phosphotransferase system of bacteria. Microbiol Rev 49:232–269Google Scholar
  11. Saier Jr, MH, Bromberg FG, Roseman S (1973) Characterization of constitutive galactose permease mutants in Salmonella typhimurium. J Bacteriol 113:512–514Google Scholar
  12. Teixeira de Mattos MJ (1984) The metabolic response of Klebsiella aerogenes to anaerobic nutrient-limited environments. A chemostat study. PhD Thesis, Elinkwijk, UtrechtGoogle Scholar
  13. Teixeira de Mattos MJ, Tempest DW (1983) Metabolic and energetic aspects of the growth of Klebsiella aerogenes NCTC418 on glucose in anaerobic chemostat culture. Arch Microbiol 134:80–85Google Scholar
  14. Tempest DW, Neijssel OM (1984) The status of Y ATP and maintenance energy as biologically interpretable phenomena. Ann Rev Microbiol 38:459–486Google Scholar
  15. Thienen GM van, Postma PW, Dam K van (1979) Proton movements coupled to sugar transport via the galactose transport system in Salmonella typhimurium. Eur J Biochem 73:521–527Google Scholar
  16. West I, Mitchell P (1973) Stoichiometry of lactose-proton symport across the plasma membrane of Escherichia coli. Biochem J 132:587–592Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. Driessen
    • 1
  • P. W. Postma
    • 1
  • K. van Dam
    • 1
  1. 1.Laboratory of BiochemistryUniversity of AmsterdamAmsterdamThe Netherlands

Personalised recommendations