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Archives of Microbiology

, Volume 146, Issue 4, pp 358–361 | Cite as

Energetics of glucose uptake in Salmonella typhimurium

  • M. Driessen
  • P. W. Postma
  • K. van Dam
Original Papers

Abstract

We have studied the energetics of glucose uptake in Salmonella typhimurium. Strain PP418 transprots glucose via the phosphoenolpyruvate: glucose phosphotransferase system, while strain PP1705 lacks this system and can only use the galactose permease for glucose uptake. These two strains were cultured anaerobically in glucose-limited chemostats. Both strains produced ethanol and acetate in equimolar amounts but a significant difference was observed in the molar growth yield on glucose (YGlc). It is suggested that this difference is due to a difference in the energetics of the glucose uptake systems in the two strains.

Assuming an equal YATP for both strains, we could calculate that uptake of 1 mole of glucose via the galactose permease consumes the equivalent of 0.5 mole of ATP. With the additional assumption that one proton is transported in symport with one glucose molecule, these results imply a stoichiometry of two protons per ATP hydrolysed.

Key words

Growth yield Energetics (of growth) Salmonella typhimurium Glucose uptake 

Abbreviations

PTS

Phosphoenolpyruvate: carbohydrate phosphotransferase system

D

dilution rate (h-1

DW

dry weight

GalP

galactose permease

EtOH

ethanol

HAc

acetate

Lact

lactate

Suc

succinate

HFo

formate

Glc

Glucose

YGlc, YATP

yield of cells per glucose or ATP

q

specific production rate

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. Driessen
    • 1
  • P. W. Postma
    • 1
  • K. van Dam
    • 1
  1. 1.Laboratory of BiochemistryUniversity of AmsterdamAmsterdamThe Netherlands

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