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Current Genetics

, Volume 10, Issue 9, pp 643–646 | Cite as

Clones from two different genomic regions complement the cdc25 start mutation of Saccharomyces cerevisiae

  • Jacques Daniel
  • Giora Simchen
Original Articles

Summary

We have cloned the CDC25 gene of Saccharomyces cerevisiae whose product is required for traversing the Go phase of the cell cycle. A preliminary physical characterization of the CDC25 gene region is presented. In addition, we show that another gene, when cloned in a high-copy number plasmid, is able to partially suppress growth thermosensitivity of a strain carrying the cdc25 mutation. We briefly discuss the possible interation of these gene products with adenylate cyclase encoded by the CDC35 gene.

Key words

Cloning Cell cycle control Start mutation Adenylate cyclase pathway 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Jacques Daniel
    • 1
  • Giora Simchen
    • 1
  1. 1.Department of Biochemistry, The Weizmann Institute of Science, Rehovot 76100 and Department of GeneticsThe Hebrew UniversityJerusalemIsrael

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