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Nitrogenase activity in the blue-green alga Plectonema boryanum strain 594

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The non-heterocystous filamentous blue-green alga, Plectonema boryanum strain 594 reduces acetylene to ethylene, incorporates 15N2 into cell protoplasm, and grows readily in medium free of combined nitrogen, when incubated in a gas phase without added oxygen. Cells grown in the presence of 50 mg/l of ammonium-nitrogen do not reduce acetylene, and a concentration of 0.015 atm. CO in the gas phase inhibits nitrogenase activity completely but inhibits 14CO2 incorporation by only 28%. Nitrogenase activity is inhibited after 2 h treatment with 3×10-5 M DCMU and is inhibited completely in air.

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Stewart, W.D.P., Lex, M. Nitrogenase activity in the blue-green alga Plectonema boryanum strain 594. Arch. Mikrobiol. 73, 250–260 (1970).

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  • Oxygen
  • Nitrogen
  • Ethylene
  • Acetylene
  • DCMU