Archiv für Mikrobiologie

, Volume 71, Issue 3, pp 283–294 | Cite as

The isolation of mutants not accumulating poly-β-hydroxybutyric acid

  • H. G. Schlegel
  • R. Lafferty
  • I. Krauss


Five mutant strains of Hydrogenomonas H 16 which synthesize poly-β-hydroxybutyric acid either slowly or not at all have been isolated following nitrite and NMG treatment of wild type cells. When grown on a nitrogen deficient agar medium, the colonies of PHB-free cells can be recognized by their diminished retention of the dye sudanblack B. Enrichment procedures for such mutants have been devised employing the 32P-phosphate inactivation technique and sucrose gradient centrifugation. The mutants have been characterized with respect to their growth properties, respiratory control and other properties.


Nitrogen Sucrose Centrifugation Agar Nitrite 
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Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • H. G. Schlegel
    • 1
    • 2
  • R. Lafferty
    • 1
    • 2
  • I. Krauss
    • 1
    • 2
  1. 1.Institut für Mikrobiologie der Gesellschaft für Strahlenforschung mbH MünchenMunichGermany
  2. 2.Institut für Mikrobiologie der Universität GöttingenGöttingenGermany

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