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Effect of disulfiram on the alkylation of rat liver DNA by nitrosodiethylamine

  • N. Frank
  • D. Hadjiolov
  • B. Bertram
  • M. Wiessler
Short Communications

Summary

The extent of ethylation of guanine in 7 N and O 6 position in rat liver DNA was studied after chronic feeding with unlabelled nitrosodiethylamine followed by a single application of the 14C labelled drug. Disulfiram inhibits the alkylation of rat liver DNA. It was also shown that a discontinuous dosage of disulfiram protects against the alkylating effect of 14C nitrosodiethylamine only for a distinct period of time.

Key words

Nitrosodiethylamine Disulfiram Alkylation DNA Metabolism 

Abbreviations

DSF

Disulfiram

NDEA

Nitrosodiethylamine

DNMA

Nitrosodimethylamine

Der Einfluß von Disulfiram auf die Alkylierung von Rattenleber DNA durch Nitrosodiäthylamin

Zusammenfassung

Die Alkylierungsrate von Guanin der Rattenleber DNA in 7 N-und O-6-Stellung wurde untersucht nach chronischer Gabe von unmarkiertem Nitrosodiäthylamin und einer abschließenden Dosis 14C markierter Substanz. Disulfiram vermindert die Alkylierungsrate in der DNA der Rattenleber. Es konnte ferner gezeigt werden, daß eine nicht fortlaufende Verabreichung von Disulfiram nur eine bestimmte Zeitdauer vor der Alkylierung durch 14C-Nitrosodiäthylamin schützt.

Schliisselwörter

Nitrosodiäthylamin Disulfiram Alkylierung DNA Metabolismus 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • N. Frank
    • 1
  • D. Hadjiolov
    • 2
  • B. Bertram
    • 1
  • M. Wiessler
    • 1
  1. 1.Institute of Toxicology and ChemotherapyGerman Cancer Research CenterHeidelbergFederal Republic of Germany
  2. 2.Institute of OncologySofia-DarvenitzaBulgaria

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